This randomized controlled trial aimed to quantify the effects of choir singing on emotional state and anxiety levels of singers. Salivary amylase, PANAS (Positive and Negative Affect Schedule) and STAIs/-t(State-Trait Anxiety Inventory) were applied before and after a 1-hour single choir session for an experimental group (n = 35) and unstructured time for control group (n = 35). Amylase decreased in the experimental group but increased in the control group (p > 0.05 for F group ; p = 0.014 for F testbygroup ). Follow-up analysis showed this interaction to be due to baseline differences between the two groups. Negative affect decreased in the experimental group and increased in the control group (p > 0.05 for F test and F group ; p = 0.006 for F test by group ). A decrease in positive affect was found between the pre-and post-test for the control group (p = 0.023 for F test ; p = 0.004 for F test by group ). State anxiety decreased in the experimental group and increased in the control group (p > 0.05 for F test and F group ; p = 0.001 for F test by group ). Singing in a choir was found to have a positive impact on psychological indicators of affect and anxiety, however, its physiological effect could not be shown using salivary amylase in this study.