Rapid and reliable detection of severe acute respiratory syndrome coronavirus 2 mutations are significant to control the contagion and spread rate of the virus. We aimed to evaluate the N501Y mutation rate in randomly chosen positive patients with the polymerase chain reaction (PCR). The evaluation and analysis of the data with a retrospective approach in cases with mutations, in terms of public health, will contribute to the literature on the global pandemic that affects our society. Public health authorities will take the necessary precautions and evaluate the current situation. The N501Y mutation was detected in patients with positive Covid-19 PCR test results. The positive samples were examined based on the 6-carboxyfluorescein (FAM) channel in reverse transcription PCR (RT-PCR) quantitation cycle (Cq) values as low Cq (<25), medium Cq (25-32), and high Cq (32-38) groups. In the study, 2757 (19.7%) of 13 972 cases were detected as mutation suspects and 159 (5.8%) of them were found to have mutations. The ages of the cases with mutations ranged from 1 to 88 years (mean age of 40.99 ± 17.55). 49.7% (n = 79) of the cases with mutations were male, and 50.3% (n = 80) were female. When the RT-PCR-Cq results were examined, it was seen that it varied between 11.3 and 35.03, with an average of 20.75 ± 3.32. K E Y W O R D S coronavirus, N501Y mutation, polymerase chain reaction (PCR), reverse transcription PCR (RT-PCR), SARS-CoV-2 alpha, gamma, and delta-coronavirus. 6 SARS-CoV-1, SARS-CoV-2, and MERS-CoV are the type beta of coronavirus and only affected on

In late 2019 on 11th March 2020 a novel coronavirus, SARS-CoV-2 causing Coronavirus disease 2019 appeared in Wuhan, China and World Health Organisation declared it to have developed pandemic status. Although there are many detection techniques for the disease to control the pandemic such as RT-PCR, serological methods, or fast antigen tests, the actual problem is the prevention of the disease. The nasal cavity and rhino pharynx are key sites of the initial replication of SARS-CoV-2. In this article, the effectiveness of destrovir spray was investigated by creating a mechanical barrier in the nasal and oral mucosa, which are the entry points of the virus into the body, and to destroy the virus that comes into contact with this barrier. In the presented study, quantitation cycle (Cq) and irradiation values (RFU) of 12 different high-positive patients (Cq≤25) after application of both RT-PCR and destrovir spray were determined at different concentrations (10%, 20%, 30%, 40%, and 50%) aimed to evaluate its effectiveness. According to the results obtained by comparing the effectiveness of disinfectant with 70% ethyl alcohol on the 46 patient samples with Cq<20, 46 patient samples with Cq values between 20-25, and 46 patient samples with Cq≥25 including different variants are examined. A total of 138 COVID-19 patient samples were treated with 5% and 10% ratios of destrovir spray. Its effectiveness on Cq values and RFU was evaluated and it was aimed to compare the effectiveness of this evaluation with 96% ethyl alcohol used in the same samples.

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Background: Nowadays, people have faced with a pandemic called COVID-19. The reliable detection of the virus is important to prevent transmission of the virus. RT-PCR is a gold standard method for the diagnosis of the disease used at all over the world. The highest number of sample size (1,461,258 patient sample) and differing results are reported with our study regarding the PCR positivity rates. Method/Study Design:The study was aimed to evaluate the positivity and negativity of the patients with RT-PCR from all the samples studied between March 25, 2020 and March 25, 2021, when the pandemic was declared and started to be seen in Turkey, and to investigate its contribution to the total test capacity of our country.Results/Conclusions: 1,461,258 patient is observed, and this frequency male is 58% and female is 42% of the population. The maximum number of admissions is noticed during the Autumn-2020 involved age ranged from 25 to 35. 14.6% positive result is got while the 85.4% negative result is observed. When the age distribution of COVID-19 (+) patients is evaluated, COVID-19 (+) rate is highest in the 6-15 age range, followed by the 66-75 age range and the highest COVID-19 (+) rate are November and October, respectively. Additionally, the highest COVID-19 (+) rate is in Autumn. According to the test results, it was determined that 7.5% of the male participants were COVID-19 (+) and 7.1% of the female participants were COVID-19 (+).

COVID‑19 is a devastating disease, and its control is difficult due to its high transmissibility rate and a long incubation average period (6.4 days). Additionally, more than half of the infected patients were asymptomatic young people or children. The asymptomatic virus transmission is the actual challenge to controlling the disease. Because of limited treatment options, diagnosis techniques have been the first focus all over the world, involving q-RT-PCR as a gold standard, serological tests, point of care studies, or RT-LAMP. Generally, nasopharyngeal, and oropharyngeal samples are preferred clinically as sources. However, alternative sources are being researched, particularly for healthcare professionals who have difficulty taking samples, patients who are afraid of giving samples, and pediatric patients. Herein, physiological saline has been utilized to offer an alternative source besides the swab samples for use in q-RT-PCR. In this study, 212 randomly chosen patients’ samples were studied, and we evaluated the concordance and accurate q-RT-PCR results in two different sources, obtained from swab and gargle samples of patients. Herein, physiological saline is utilized, which is widely used medically as a recommended irrigating and wound dressing solution. We obtained in our experiments with this method, the confidence interval determines 74.50% positivity when compared to the routine q-RT-PCR procedure as summarized. In addition, when only the gargle sampling method is studied in low-income countries, the cost of testing for COVID-19 will decrease significantly. Because this method does not require vNAT or VTM transport solution sterile swab sticks as shown. The plastic container with a lid in which the patient can gargle with SF and spit it out is an ideal method for this. Additionally, it provides a great cost-benefit in low-income countries.