Background: Left ventricular ejection fraction (LVEF) is a strong prognostic parameter in patients with heart disease. The assessment of global longitudinal strain (GLS) from speckle-tracking analysis of 2-dimensional echocardiography has become a clinically feasible alternative to LVEF for the measurement of myocardial function. Objective: The aim of the current work was to compare between GLS speckle tracking echocardiography and Simpson's biplane methods for assessment of left ventricular function in non-STEMI patients. Patients and methods: This study was carried out in Cardiology Department, Faculty of Medicine, Zagazig University on 44 non-ST-elevation myocardial infarction (NSTEMI) patients scheduled for assessment of Left ventricular function by GLS and biplane Simpson method. Result: Mean systolic blood pressure was 122.05±18.37 mmHg. No statistically significant difference in systolic blood pressure was found across GLS groups (P= 0.17). Mean diastolic blood pressure was 73.41±12.00 mmHg. No statistically significant difference in diastolic blood pressure was found across GLS groups (P= 0.35). Mean heart rate was 68.84±7.13 bpm, and a statistically significant difference was found in heart rate across GLS groups (P= 0.039). Conclusion:It could be concluded that GLS speckle tracking echocardiography and Simpson's biplane methods can be used as alternative different parameters for assessment of left ventricular function in non-STEMI patients.