Purpose Eri silk fiber has superior thermal insulation behavior, better softness than cotton fiber. However, Eri silk’s use in the commercial arena has not yet taken off. The purpose of the study is to explore the comfort properties of the fabric, which enhances the commercial acceptance of Eri silk clothing. Design/methodology/approach In this investigation, three different single knit Eri silk structures were produced with different loop lengths and yarn counts to analyze the influence of process variables on low-stress mechanical properties. To execute the research work, Eri silk spun yarn of three different linear densities (15 tex, 20 tex, 25 tex) were chosen. Three different knitted structures were produced, such as single jersey, popcorn and cellular blister, and two different loop lengths were also selected. Findings The cellular blister structure has shown appreciable low-stress properties next highest position was attained by the popcorn structure. Yarn fineness and loop length were significant with most of the low-stress properties. Research limitations/implications The findings of this research will contribute to a greater awareness of Eri silk knitted fabric and its process parameters in relation to its commercial utility. Originality/value This study was conducted to explore the influence of knit structure, loop length and yarn count on the low-stress properties of Eri silk-based thermal clothing.