Abstract. Groundwater-dependent ecosystems (GDEs) are at risk globally due to unsustainable levels of groundwater extraction, especially in arid and semi-arid regions. In this review, we examine recent developments in the ecohydrology of GDEs with a focus on three knowledge gaps: (1) how do we locate GDEs, (2) how much water is transpired from shallow aquifers by GDEs and (3) what are the responses of GDEs to excessive groundwater extraction? The answers to these questions will determine water allocations that are required to sustain functioning of GDEs and to guide regulations on groundwater extraction to avoid negative impacts on GDEs.We discuss three methods for identifying GDEs: (1) techniques relying on remotely sensed information; (2) fluctuations in depth-to-groundwater that are associated with diurnal variations in transpiration; and (3) stable isotope analysis of water sources in the transpiration stream.We then discuss several methods for estimating rates of GW use, including direct measurement using sapflux or eddy covariance technologies, estimation of a climate wetness index within a Budyko framework, spatial distribution of evapotranspiration (ET) using remote sensing, groundwater modelling and stable isotopes. Remote sensing methods often rely on direct measurements to calibrate the relationship between vegetation indices and ET. ET from GDEs is also determined using hydrologic models of varying complexity, from the White method to fully coupled, variable saturation models. Combinations of methods are typically employed to obtain clearer insight into the components of groundwater discharge in GDEs, such as the proportional importance of transpiration versus evaporation (e.g. using stable isotopes) or from groundwater versus rainwater sources.Groundwater extraction can have severe consequences for the structure and function of GDEs. In the most extreme cases, phreatophytes experience crown dieback and death following groundwater drawdown. We provide a brief review of two case studies of the impacts of GW extraction and then provide an ecosystem-scale, multiple trait, integrated metric of the impact of differences in groundwater depth on the structure and function of eucalypt forests growing along a natural gradient in depth-to-groundwater. We conclude with a discussion of a depth-to-groundwater threshold in this mesic GDE. Beyond this threshold, significant changes occur in ecosystem structure and function.

Although it is well documented that access to groundwater can help plants survive drought in arid and semiarid areas, there have been few studies in mesic environments that have evaluated variation of vegetation characteristics across a naturally occurring gradient in depth-to-groundwater (DGW). The aim of this study was to determine whether differences in groundwater depth influence structural attributes and productivity of remnant woodlands in south-eastern Australia. The study area was located in the Kangaloon bore-field area of New South Wales, where DGW varies from 2.4 m to 37.5 m and rainfall is plentiful. We examined structural (leaf-area index, basal area, stem density, tree height, Huber value (HV) and aboveground biomass) and functional (aboveground net primary productivity (ANPP)) attributes of seven woodland sites differing in DGW. We also used ∂13C analysis of sapwood across six sites, along with observed non-linear changes in structural attributes, to infer groundwater use by trees. Significant differences in structural attributes and ANPP were observed across sites. The three shallowest sites with 2.4 m, 4.3 m and 5.5 m DWG had significantly larger aboveground biomass and ANPP than did the four deepest sites (DGW ≥9.8 m). Across all attributes (except HV in the summer, where the mean values were significantly larger at sites where DGW was 5.5 m or less and across the four deeper sites (DGW ≥9.8 m), there were no differences in these three structural traits, nor in ANPP. Despite finding no significant differences in HV across sites in the summer, in winter, the two deepest sites had a significantly larger HV than did the two shallowest sites. Significant increases in ∂13C of sapwood occurred across five of the six sites, consistent with increasing water-use efficiency as DGW increased, reflecting the declining availability of groundwater with increasing DGW. This study has demonstrated that even in a mesic environment, putative access to groundwater can have important impacts on structural and functional traits of trees and, consequently, on woodland productivity.

Abstract. Groundwater-dependent ecosystems (GDEs) are at risk globally due to unsustainable levels of groundwater extraction, especially in arid and semi-arid regions. In this review, we examine recent developments in the ecohydrology of GDEs with a focus on three knowledge gaps: (1) how do we locate GDEs, (2) how much water is transpired from shallow aquifers by GDEs; and (3) what are the responses of GDEs to excessive groundwater extraction? The answers to these questions will determine water allocations that are required to sustain functioning of GDEs and to guide regulations on groundwater extraction to avoid negative impacts on GDEs. We discuss three methods for identifying GDEs: (1) fluctuations in depth-to-groundwater that are associated with diurnal variations in transpiration, (2) stable isotope analysis of water sources in the transpiration stream; and (3) remote sensing methods. We then discuss several methods for estimating rates of GW use, including direct measurement using sapflux or eddy covariance technologies, estimation of a climate wetness index within a Budyko framework, spatial distribution of ET using remote sensing, groundwater modelling and stable isotopes. Remote sensing methods often rely on direct measurements to calibrate the relationship between vegetation indices and ET. ET from GDEs is also determined using hydrologic models of varying complexity, from the "White method" to fully coupled, variable saturation models. Combinations of methods are typically employed to obtain clearer insight into the components of groundwater discharge in GDEs, such as the proportional importance of transpiration vs. evaporation (e.g., using stable isotopes) or from groundwater vs. rainwater sources. Groundwater extraction can have severe consequences on structure and function of GDEs. In the most extreme cases, phreatophytes experience crown dieback and death following groundwater drawdown. We provide a brief review of two case studies of the impacts of GW extraction and discuss the use of C isotope ratios in xylem to reveal past influences of GW extraction. We conclude with a discussion of a depth-to-groundwater threshold in mesic and semi-arid GDEs. Across this threshold, significant changes occur in ecosystem structure and function.