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remained unchanged for over a decade, and early recognition continues to be the most crucial factor in survival outcome. Early and accurate diagnosis of infection and organ dysfunction remains problematic, as evidenced by numerous interventional trials that have not resulted in improved outcomes. These failures are partly because of the belated intervention, when the patient developed multiple-organ failure and the therapeutic window of opportunity closed. The success of immunomodulatory and other therapeutic strategies, which is often achieved in preclinical models of sepsis, depends on their use in the early stages of sepsis development or even proactive action. Predicting the development of sepsis in surgical patients using laboratory analysis of plasma may be useful for doctors in the intensive care unit and resuscitation. Significant efforts are being made to develop biomarkers for the early stages of sepsis with high sensitivity and specificity. For early and accurate diagnosis, effective treatment of sepsis requires a deep understanding of the pathogenetic mechanisms. Dysregulation of the patients response to infection leading to sepsis and septic shock is studied using ohmic approaches: proteomics, transcriptomics, and metabolomics. Owing to the complexity and large volume of data sets, special data analysis tools, the so-called machine learning, become necessary.

The objective: to study in vitro adsorption properties of various devices for selective lipopolysaccharide (LPS) adsorption.Subjects and Methods: Various methods of closed circuit circulating bovine serum endotoxin solutions were used. The serum was perfused using an LPS sorption device for 240 min. Serum samples were collected before the start of perfusion, and 30, 60, 120, 150, and 240 minutes after the start of circulation. LPS concentrations were measured by the turbidimetric method. One column for polymyxin hemoperfusion and three devices for selective adsorption of lipopolysaccharides were assessed.Results: When using the device for polymyxin hemoperfusion, the concentration of endotoxin in bovine serum decreased by 61% during 120 minutes, and in 120 minutes after additional administration of endotoxin, it went down by 57%. When using the other three devices, these parameters made 9% and 6%, 10% and 8%, 5% and 10%, respectively. Conclusion. By definition, an in vitro study cannot provide for complex pathophysiological reactions occurring in the body during sepsis. Тhis fact leads to limitations in extrapolating the results obtained to clinical practice.