Citrus exports to foreign markets are severely limited today by fruit diseases.Some of them, like citrus canker, black spot and scab, are quarantine for the markets. For this reason, it is important to perform strict controls before fruits are exported to avoid the inclusion of citrus affected by them. Nowadays, technical decisions are based on visual diagnosis of human experts, highly dependent on the degree of individual skills. This work presents a model capable of automatic recognize the quarantine diseases. It is based on the combination of a feature selection method and a classifier that has been trained on quarantine illness symptoms. Citrus samples with citrus canker, black spot, scab and other diseases were evaluated. Experimental work was performed on 212 samples of mandarins from a Nova cultivar. The proposed approach achieved a classification rate of quarantine/not-quarantine samples of over 83% for all classes, even when using a small subset (14) of all the available features (90). The results obtained show that the proposed method can be suitable for helping the task of citrus visual diagnosis, in particular, quarantine diseases recognition in fruits.
Exposure of citrus fruit to frost often results in the development of freeze injury during their maturation in planta. This work was aimed to analyze changes in the biochemistry and enzymology of carbohydrate metabolism in freezeinjured orange fruit (Citrus sinensis var. Valencia late) and the involvement of oxidative stress in frost damage. The activities of pyrophosphate-dependent phosphofructokinase, phosphoenolpyruvate carboxylase and several fermentative enzymes increased in frost-exposed (FE) fruit, while NADP-malic enzyme and the mitochondrial isoform of NAD-malate dehydrogenase showed a reduction in their activities. Western blot analysis indicated a correlation between activity levels and protein content. Respiration rate in whole fruit was reduced by 40%, whereas the flavedo showed a more pronounced decline (53%). Volatile compound (i.e. ethanol and acetaldehyde) content was significantly higher in FE fruit than in control, as was that of L-malate (three-fold). Additionally, FE fruit showed a marked decrease in the maturity index (24%) because of a higher titratable acidity (39%). Evidence is presented that oxidative stress is involved in freeze-induced damage of orange fruit, where oxidative damage to lipids and proteins, and a greater electrolyte leakage in the flavedo were also observed. The results suggest that freezing temperatures provoke a notable metabolic switch in citrus fruit toward a fermentative stage, resulting in low-quality fruits.
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