-Objective The purpose of this study is to analyze the results of 661 amniocentesis cases performed for prenatal diagnosis at a maternity hospital. -Methods This is a retrospective study for a one year period (2008-2009) of case notes of 661 pregnant women who undergone amniocentesis for prenatal diagnosis and chromosomal abnormalities. Data expressed as actual counts and percentages -Results: The reasons for having amniocentesis were as follows: high risk in triple screening test (44.8%), advanced maternal age (44.5%), abnormal ultrasonographic examination (5.9%), history of previous births with down syndrome or abnormalities (3.3%), history of chromosomal abnormalities in parents (1.5%). The success rate of cytogenetic analysis was 100.0%. Chromosomal abnormalities were observed in 4.2% of the patients. -Conclusion: Among those with abnormal ultrasonographic examination chromosomal abnormalities were found to be higher than those with high risk triple test or advanced mother age. Structural chromosomal abnormalities were found lesser than the numerical abnormalities. High risk in triple screening, advanced maternal age and abnormal ultrasonographic results were three major indications for amniocentesis respectively. Chromosome abnormalities were detected in 4.2% of cases and most of these abnormalities were found in cases with abnormal ultrasonography. [TAF Prev Med Bull 2012; 11(1.000): 19-22