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Background: Demodex mites can cause ocular demodicosis in humans. Natural products such as tea tree oil are used for ocular demodicosis, which can take a long time to treat. The aim of our study is to investigate the effects of some essential oils on in vitro mite lifespan. Methods: Eyelashes were examined under a light microscope. Mites were determined and the killing times of mineral oil, tea tree oil, black seed oil, St. John's Wort oil, sage oil and thyme oil were recorded. The active ingredients of thyme, tea tree and sage oils were determined by GC / MS method.Results: It was determined that 1% concentration of thyme oil has more effective killing time than 5% concentrations of tea tree oil and sage oil (p <0.0001). The most important active ingredients were carvacrol in thyme oil, terpinen-4-ol in tea tree oil, and 1.8-cineol in sage oil. Conclusion: Thyme oil was the most effective oil in killing mites. In addition, the first steps were taken that thyme oil and its products can be used in therapy.

Purpose: Demodex folliculorum and Demodex brevis are common ectoparasites on skin that also can lead to blepharitis and chalazion. The aim of our study is to determine the prevalence of Demodex spp. in eyelashes of patients diagnosed with chronic blepharitis and chalazion.Methods: This study included 330 patients diagnosed with chronic blepharitis, 70 patients diagnosed with chalazion and 130 volunteers without any ocular problems. Patient eyelashes were examined under a light microscope at magni cations of × 40, × 100 and × 400. Demodex spp. were determined.Results: Parasite prevalence was signi cantly higher in blepharitis (75.5%) and chalazion groups (70%) compared to the control group (16.2%) (p <0.001). The prevalence of D. folliculorum in the blepharitis group and D. brevis in the chalazion group was found to be signi cantly higher compared to other groups (p <0.05). The average number of mites per eyelash was found to be signi cantly higher in patients with Demodex positive blepharitis (p = 0.001) and in chalazion patients (p = 0.047) than in the control group. It has been determined that mite positivity increases with age in blepharitis and control groups (p <0.05). In the group with blepharitis, it was found that mite positivity was signi cant in the presence of symptoms (p = 0.0001) and Demodex positivity decreased as the education level of individuals increased (p = 0.039). Conclusion:The results of the study show that Demodex acari infestations should be considered in chronic blepharitis and chalazion.

Purpose: Demodex folliculorum and Demodex brevis are common ectoparasites on skin that also can lead to blepharitis and chalazion. The aim of our study is to determine the prevalence of Demodex spp. in eyelashes of patients diagnosed with chronic blepharitis and chalazion. Methods: This study included 330 patients diagnosed with chronic blepharitis, 70 patients diagnosed with chalazion and 130 volunteers without any ocular problems. Patient eyelashes were examined under a light microscope at magnifications of × 40, × 100 and × 400. Demodex spp. were determined. Results: Parasite prevalence was significantly higher in blepharitis (75.5%) and chalazion groups (70%) compared to the control group (16.2%) (p <0.001). The prevalence of D. folliculorum in the blepharitis group and D. brevis in the chalazion group was found to be significantly higher compared to other groups (p <0.05). The average number of mites per eyelash was found to be significantly higher in patients with Demodex positive blepharitis (p = 0.001) and in chalazion patients (p = 0.047) than in the control group. It has been determined that mite positivity increases with age in blepharitis and control groups (p <0.05). In the group with blepharitis, it was found that mite positivity was significant in the presence of symptoms (p = 0.0001) and Demodex positivity decreased as the education level of individuals increased (p = 0.039). Conclusion: The results of the study show that Demodex acari infestations should be considered in chronic blepharitis and chalazion.

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