In this project, facile, rapid biosynthesis and characterization of silver-montmorillonite (MMT) nanocomposite is carried out at ambient temperature. Silver nanoparticles (Ag-NPs) were prepared by water extract of the plant [Satureja hortensis (L)] as the reducing agent and MMT as interlamellar space for controlling the size of Ag-NPs. MMT was sonicated in the aqueous AgNO 3 solution and Ag + ions were reduced to Ag° using water extract of Satureja hortensis (L). The nanocomposite was evaluated using ultraviolet-visible spectroscopy (UV-Vis), X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). TEM study showed the formations of nanocomposite using water extract of Satureja hortensis L in the range of 4.88-26.70 nm and an average particles size of 15.79 nm. In addition, XRD studies indicated that the particles have a face centred cubic (FCC) structure. The nanocomposite showed antibacterial effects against bacteria.

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