RESUMOAvaliaram-se dois protocolos para a produção de plasma rico em plaquetas (PRP) com o sangue de 20 cães adultos. Foram coletados três frascos de sangue em que um deles foi usado para produção do PRP por meio do protocolo A − centrifugação única a 1200rpm/10min −, o outro para fabricação do PRP pelo protocolo B − primeira centrifugação a 1200rpm/10min e a segunda centrifugação a 1600rpm/10min − e o terceiro para realização da contagem plaquetária no sangue total, que serviu de parâmetro para os valores alcançados no PRP. O protocolo no qual foi possível alcançar maior concentração plaquetária foi testado em outros 20 cães para avaliar sua reprodutibilidade. Constatou-se que o protocolo B resultou em maior plaquetometria em 100% das amostras e concluiu-se ser ele eficiente para a produção do PRP em cães.Palavras-chave: cão, fatores de crescimento, gel de plaquetas ABSTRACT The objective of this paper was to analyze two protocols for the production of platelet rich plasma (PRP) in dogs. Peripheral blood of 20 adult dogs was collected into three tubes. The first was processed throughprotocol A − single centrifugation at 1200rpm for 10min −, the second was submitted to protocol B -a first centrifugation at 1200rpm for 10min and a second centrifugation at 1600rpm for 10min − and the third was used to perform platelet count in whole blood, which served as a parameter for values obtained in PRP. The protocol in which it was possible to achieve a higher platelet count was tested in other 20 dogs to evaluate its reproducibility. Protocol B resulted in a superior platelet count in 100% of the samples, concluding that the referred protocol is effective for PRP production in dogs.

Background There is accumulating evidence that extent of resection (EOR) in intrinsic brain tumor surgery prolongs overall survival (OS) and progression-free survival (PFS). One of the strategies to increase EOR is the use of intraoperative MRI (ioMRI). However, considerable infrastructure investment is needed to establish and maintain a sophisticated ioMRI. We report the preliminary results of an extra-operative (eoMRI) protocol, with a focus on safety, feasibility and EOR in intrinsic brain tumor surgery. Methods Ten patients underwent an eoMRI protocol consisting of surgical resection in a conventional operating room followed by an immediate MRI in a clinical MRI scanner while the patient was still under anesthesia. If MRI suggested residual safely resectable tumor, the patient was returned to the operating room. Retrospective volumetric analysis was undertaken to investigate the percentage of tumor resected after first resection and if applicable, after further resection. Results 6 out of 10 (60%) patients were felt to require no further resection after eoMRI. The EOR in these patients was 97.8±1.8%. In the 4 patients who underwent further resection, the EOR during the original surgery was 88.5±9.5% (p =0.04). There was an average of 10.1 % more tumor removed between the first and second surgery. In 3/4 (75%) of patients who returned for further resection, gross total resection of was achieved. Conclusion An eoMRI protocol appears to be a safe and practical method to ensure maximum safe resections in patients with brain tumors and can be performed readily in all centers with MRI capability.