Basis gigi tiruan adalah bagian yang berkontak langsung dengan jaringan lunak rongga mulut. Bahan basis gigi tiruan yang paling umum digunakan yaitu resin akrilik polimerisasi panas. Bahan ini masih memiliki kekurangan yaitu sifat mekanis rendah, meninggalkan monomer sisa dan adanya porositas yang berpengaruh terhadap kekasaran permukaan. Nanoselulosa sekam padi memiliki potensi sebagai alternatif untuk memperbaiki sifat bahan resin akrilik polimerisasi panas. Nanoselulosa memiliki keunggulan diantaranya memiliki luas permukaan yang tinggi sehingga dapat digunakan sebagai reinforcing nanofiller pada material komposit seperti resin akrilik. Tujuan penelitian ini adalah untuk mengetahui pengaruh penambahan nanoselulosa sekam padi terhadap kekasaran permukaan pada resin akrilik polimerisasi panas. Jenis penelitian ini adalah eksperimental laboratoris dengan post-test only control group design. Sampel penelitian terdiri dari 6 kelompok, masing-masing kelompok terdiri dari 8 sampel. Kelompok K1, K2, K3, K4, K5 dan K0 berturut-turut merupakan kelompok dengan penambahan nanoselulosa 1%, 2%, 3%, 4%, 5% serta kontrol (tanpa nanoselulosa). Hasil penelitian menunjukkan semua kelompok dengan penambahan nanoselulosa memiliki nilai kekasaran permukaan lebih tinggi dibanding kelompok kontrol. Penambahan nanoselulosa 5% (K5) memiliki nilai kekasaran permukaan tertinggi yaitu 0,3242 ± 0.0066µm kemudian diikuti berturut-turut oleh kelompok K4, K3, K2, K1 dan kelompok kontrol (K0) memiliki nilai kekasaran permukaan terendah yaitu 0.1558 ± 0.0023µm. Hasil uji SEM pada setiap kelompok menunjukkan terdapat aglomerasi dan ukuran porus yang bervariasi. Kesimpulan penelitian ini adalah penambahan nanoselulosa sekam padi tidak lebih baik dari kelompok kontrol dan dapat meningkatan kekasaran permukaan basis gigi tiruan resin akrilik polimerisasi panas.

Background: Microleakage during restoration causes secondary caries. The shrinkage of nanohybrid composite resin can occur during the polymerization process, affected by both the selection of bonding materials featuring different solvents, such as ethanol and isopropanol, as well as contrasting conditions such as moist and dry. Purpose: This study aimed to determine and analyze the differences of microleakage level of nanohybrid composite resin using the eighth-generation bonding materials made from ethanol and isopropanol solvents under moist and dry conditions. Methods: This research constituted an experimental laboratory study. The samples were divided into four groups. Group I used a bonding material produced from ethanol under dry conditions. Group II used a bonding material produced from ethanol under moist conditions. Group III used a bonding material produced from isopropanol under dry conditions. Group IV used a bonding material produced from isopropanol under moist conditions. The levels of microleakage were subsequently tested using a stereo microscope. Results: Microleakage examination was performed by means of a stereo microscope to observe the methylene blue color penetration with assessment subsequently being performed on a scale of 0 to 3. The statistical results of a Kruskal-Wallis test showed that no significant differences occurred in any of the treatment groups (sig = 0.141, p<0.05). Conclusion: There was no difference in the microleakage levels of nanohybrid composite resin using eighth-generation bonding materials produced from ethanol and isopropanol solvents under moist and dry conditions.

Introduction: Tooth discolouration is the most common esthetic problems in dentistry. Direct composite veneer is an adjunctive therapy for severe teeth discolouration by attaching to the tooth surface using an acid etching and dental bonding agent. In several cases of severe discolouration, it is required to have certain preliminary treatments such as bleaching prior to the placement of the composite veneer. However, bleaching agents are able to lead the enamels morphology and microstructure alteration by inhibiting the polymerisation bonding thus affecting the veneer adhesion. This study was aimed to determine the tensile bond strength of direct composite veneers between the V and VII generation dentin bonding agent after extracoronal bleaching. Methods: This study was an experimental laboratory of as much as 32 human permanent maxillary central incisors were divided into 4 groups. Group 1 was treated with extracoronal bleaching and bonded with the V generation bonding nanohybrid composite. Group 2 was treated with extracoronal bleaching and bonded with the VIII generation bonding nanohybrid composite. Group 3 was only bonded with the V generation bonding nanohybrid composite. Group 4 was only bonded with the VIII generation bonding nanohybrid composite. All samples were immersed in artificial saliva then incubated at the room temperature for 24 hours. Results: The tensile strength value was determined using the Universal Testing Machine. The ANOVA test results showed significant differences in all four groups (p < 0.05). Conclusion: The VIII generation bonding agent had a higher tensile strength than the V generation.

Background: Chronic periodontitis, a chronic inflammatory condition of periodontal tissue, can occur due to microorganisms as a local factor and genetic as a systemic factor. Genetic factors that cause chronic periodontitis, namely mutations in the form of a gene that functions to regulate calcium homeostasis, which is called the vitamin D receptor (VDR) gene. Mutated VDR gene causing different effect in a population and ethnic groups, including Javanese Banyumas Ethnic. Purpose: To understand the genotype distribution and VDR BsmI (rs1544410) gene allotype frequency chronic periodontitis patient in Javanese Banyumas Ethnic.  Method: The research was descriptive research in the form of a cross-sectional study of 26 chronic periodontitis patients in the Javanese Banyumas tribe. The samples obtained were carried out by the process of DNA isolation, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), and data in the Hardy-Weinberg Equilibrium (HWE) Court Lab analysis. Result: Whole samples from DNA isolation amplificated with one ribbon 524 bp VDR gene. RFLP restriction enzyme Bsml process is also showing cutting in each sample. From data analysis, X2 is 0,44 with p=0,50 (p>0,05) resulted. It was showing mutant VDR gene allele frequency in chronic periodontitis patients in Javanese Banyumas ethnic were balanced genetically. Conclusion: Genotype distribution in chronic periodontitis patient in Javanese Banyumas ethnic were 20 (76,9%) GG (bb) genotype, 6 (23,1%) GA (Bb) genotype, and 0 (0%) AA (BB) genotype with alel G (b) (wild type) allele frequency 0,88 (88%) and A allele (B or mutant) 0,12 (12%).