Bacterial exopolysaccharides
(EPSs) are important alternatives
to plant polysaccharides in fermented products and exhibit antioxidant
activity, which is particularly desirable for functional foods. This
study evaluated the use of spent media wastewater (SMW) derived from
kimchi fermentation for the production of an EPS and analyzed the
characterization and antioxidant activity of the resulting EPS. The
EPS concentration and conversion yields of sequential purification
were 7.7–9.0 g/L and 38.6–45.1%, respectively. Fourier
transform infrared spectra and NMR spectra indicated that the EPS
was a linear glucan with α-(1 → 6) linkages. The EPS
also exhibited thermal tolerance to high temperatures. In
vitro antioxidant activity analyses indicated the scavenging
activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, thiobarbituric
acid reactance (TBAR), and ferric ion reducing antioxidant power (FRAP)
values of 71.6–79.1, 28.2–33.0%, and 0.04–0.05
mM FeCl3, respectively. These results reveal that the EPS
extracted from SMW has potential as a thermally tolerant, nontoxic,
and natural antioxidant for industrial applications.
The biorefining of agricultural waste into green chemicals
has
clear potential for improving global environmental sustainability.
In this study, we evaluated the potential of acetic acid production
from carbohydrate feedstock (onion waste, OW) as a more environmentally
friendly source than feedstock produced from natural gas. In particular,
OW is an ideal feedstock for the biorefining process as it contains
a sufficient amount of carbohydrates (69.7%). Five days of the simultaneous
saccharification and two-step fermentation (SSTF) process produced
acetic acid from OW more efficiently than the simultaneous saccharification
and cofermentation (SSCF) process. SSTF produced 19.3 g/L acetic acid
and recorded the highest conversion yield (90.5%) from OW (6% substrate
loading, w/v). These results suggested that acetic acid can be efficiently
and sustainably produced from OW by the SSTF process.
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