No abstract

Due to the shortage of human organ donors for transplant, various studies of xenotransplantation, or the use of animal organs instead of human organs, have been carried out. The organs of porcine are thought to be safer and of a more suitable size for xenotransplantationthan those of nonhuman primates. Understanding the levels of expression of proteins, and their post-translational regulation, would be very practical between different species and among developing stages, though the molecular profiling for xenotransplantation has been rarely studied for porcine, while that of human and rodent is well known. Here, in this present study, we report protein regulation of the developing stages of liver (4-day old neonate, 19-day old piglet and 14-month old adult miniature pigs) using 2-DE and MALDI-TOF. From images of the three different stages, a total of 8 spotswhich were differently regulated were identified, and 5 spots were identified with MALDI-TOF MS. The data presented within this study provides critical direction relating to the development of livers of miniature pigs, which will assist future proteome analysis of the liver, and advance our understanding of the hurdles facing xenotransplantaion.

The multi-slope MUSCL, proposed by T. Buffard and S. Clain, determines slopes of conserved variables at each edge of a cell in the linear reconstructions of data. In this study, the second order accurate numerical model was developed according to the multi-slope MUSCL to solve the shallow water equations on the unstructured grids. The HLLL scheme of approximate Riemann solvers was used to calculate fluxes. For the review of the applicability of the developed model, the results of the model were compared to the 'isolated building test' and the 'model city flooding experiment' conducted as part of the IMPACT (Investigation of extreMe flood Processes And unCerTainty) project in Europe. There were limitations to predict abrupt rising of water depths by the resistance of model buildings and water depths at the specific locations among the buildings. But they were identified as the same problems also revealed in results of the other models to the same experiment. On the more refined meshes to the 'model city flooding experiment' simulated results showed good agreement with measurements. It was verified that the developed model simulated well the complex phenomena such as a dam-break problem and the urban inundation by flash floods.

No abstract