Abstract. The objective of this study was to evaluate the efficacy of intravenous infusion of hypertonic glucose for recycling of the cows with postpartum anestrus. One hundred true anestrus Holstein-Frisian cows with ≥ 60 days in milk (DIM) according to the ultrasound examinations were selected from a dairy herd located on the suburb of Tabriz. Anestrus cows randomly were allocated into two groups: A&B (n=50). In group A (Gluco-Select synch), cows received 500 mL hypertonic glucose for 3 consecutive days (-3 to 0) IV, 0.021 mg bucerelin acetate on the day 0, IM followed by 25 mg dinoprost tromethamine IM on the day 7. In group B (Select synch), the same protocol was carried out on the cows except that the glucose hypertonic solution replaced by normal saline as placebo. Thereafter all cows were detected for estrus signs for the next 5 days and inseminated based on visual signs. Twenty seven out of 50 cows (54 %) in group A and 8 out of 50 cows (16 %) in group B showed the estrus signs respectively. Conception rates were recorded as A=44.5 %, B=37.5 % for the first insemination and A=B=100 % for the second insemination of cows. The number of insemination per pregnancy was calculated as 1.55 and 1.62 for groups A and B respectively. In the case of estrus rate, the difference between two groups was statistically significant (P≤0/01), but in the cases of conception rate or the number of insemination per pregnancy rate the differences were not statistically significant.
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