No abstract

Genetic diversity was evaluated by sequencerelated amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers among 45 lemons (Citrus limon (L.) Burm. f.), five citrons (Citrus medica L.), four rough lemons (Citrus jambhiri Lush), and two Citrus volkameriana accessions. Twenty-one SRAP primer combinations produced a total of 141 (77%) polymorphic fragments with an average of 6.7 fragments per primer combinations whereas 13 SSR primers produced a total of 26 (76%) polymorphic fragments with an average of 2.0 per primer. The unweighted pair-group method arithmetic average analysis as assessed with combined SRAP and SSR data demonstrated that the accessions had a similarity range from 0.65 to 1.00. Rough lemons and C. volkameriana accessions were relatively closely related. In lemon group, accessions from hybrid origin were distant from the others. We also applied principal components analysis (PCA) for a better presentation of relation among the accessions studies. Using PCA, 88.7% of the total variation in the original dimensions could be represented by just the two dimensions defined by the first two PCs. Although nearly all accessions could be distinguished, there was a low level of genetic diversity detected among lemon cultivars.

No abstract

Total and individual tocopherol contents of sun¯ower oil, processed either by chemical re®ning (CR), physical re®ning (PR) or soft column deodorization (SCD) methods were investigated. Total tocopherol content gradually decreased until the end of the re®ning process (the deodorization stage). Among the re®ning methods used CR and PR caused higher losses in the tocopherol content when compared with the SCD method. The content of a-, b-, c-and d-tocopherols also decreased as a result of the re®ning process. These signi®cant losses in b-and c-tocopherol contents were determined after the deodorization stage.

In this study, the effects of rootstocks on storage life and quality in Gold Nugget loquat grafted on quince, hawthorn and loquat rootstocks were investigated. After harvest, fruit were placed in plastic boxes (2 kg) covered with stretch film and stored for 45 days at 5 ± 0.5°C and 90 ± 5% RH. Weight loss, fruit firmness, total soluble solid, titratable acidity, maturity rate, respiration rate, ethylene production, CO2 and O2 concentrations in package, skin colour (L*,a*,b*,C*,h°), decay rate and sensory quality of fruit were determined at 15-day intervals during storage. The same analyzes were repeated for shelf life evaluation after keeping fruit 2 days in ambient condition (20°C and 70±5 RH%). Fruit grown on quince rootstock had the best results for maintaining external appearance, titratable acidity, maturation rate and vivid skin colour. The lowest decay rate and respiration rate during storage were also obtained from this combination. Quince and loquat seedling rootstocks had similar results for sensory quality and decay rate. Covering boxes by stretch film (20 µm) reduced the weight loss in the all the combination of scion/rootstock but, increased pathogens development. These findings revealed that fruit, obtained from the combinations of Gold Nugget variety with quince and loquat seedling rootstocks, can be stored with good quality for 30+2 days at 5°C and 90 ± 5 RH%.