Treatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) contains thrombin and fibrinogen to form an adhesive fibrin clot routinely used in surgical procedures. Here, we aimed to investigate the effects of ATSCs + FS treatment on healing of PDL after tooth replantation in a rat model. After 60 min of extraction, maxillary central incisor teeth were replanted with ATSCs + FS. Two months later, the rats were sacrificed and hemimaxilla blocks were dissected out for histological analysis. The results showed that there was a significant improvement in histological findings of ATSCs + FS treated group compared to only FS treated and non-treated groups corresponding to reduced inflammatory resorption and increased new PDL formation. Furthermore, the ankylosis levels were lowered after ATSCs + FS treatment. Singular use of FS improved PDL healing moderately. Our results indicated that ATSCs + FS treatment improves PDL healing after tooth replantation suggesting a new therapeutic potential in the treatment of dental avulsion cases.

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ABSTRACTvalproic acid (2-n-propylpentanoic acid, vPA), the most widely used antiepileptic drug, has potential adverse effects and it can disrupt the oxidant and antioxidant balance. Edaravone (3-methyl-1-phenyl-2-pyrazoline-5-one, EDA) is a potent free radical scavenger. In this study, the effect of EDA on gingiva in vPA induced toxicity was investigated. female Sprague Dawley rats were randomly divided into four groups: control group, EDA (30 mg/kg/day) given group, vPA (0.5 g/kg/day) given group, and vPA+EDA (in same dose and time) given group. EDA and vPA were given intraperitoneally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and reduced glutathione (GSH) levels and catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), myeloperoxidase (mPO), alkaline phosphatase (ALP), acid phosphatase (ACP), sodium potassium ATPase (na + /K + -ATPase) and tissue factor (Tf) activities were determined in gingiva homogenates. The vPA-induced increases were statistically significant for mPO (p<0.01), ACP (p<0.01), na + /K + -ATPase (p<0.05) and Tf (p<0.01) activities, but not for LPO level and ALP activities. EDA treatment markedly blunted all such elevated anomalies. Conclusively, vPA induced oxidative and inflammatory gingival tissue damage, reactions that were appreciably reversed by concurrent administration of EDA.

Objective: Anxiety due to the dentist and dental treatment is a problem encountered in many children. The aim of the present study is to determine salivary nitric oxide, lactoferrin, α-amylase and cortisol levels of children in primary and mixed dentition, and to evaluate their relation with stress due to dental treatment. Material and Method: The study consisted of 50 children in primary and mixed dentition. The children were evaluated clinically and according to Frankl Behavior Rating Scale. Salivary flow rate was calculated, and nitric oxide, lactoferrin, α-amylase and cortisol levels were measured in saliva. Results: 68% percent of the children were found to be negative according to the Frankl Behavior Rating Scale (category 2), and significantly decreased salivary flow rate was evident in these children when compared with children that were categorized as completely negative (category 1). The DMFT+dft index was 7.56±4.29, and positive correlations were found between DMFT+dft indices and salivary nitric oxide, lactoferrin, cortisol and α-amylase levels (p<0.05). These parameters were not different between genders. Positive correlations were found between salivary nitric oxide and α amilase, cortisol and α amilase, cortisol and lactoferrin; and also between α-amilase and lactoferrin levels (p<0.05). Conclusion: Salivary lactoferrin, α-amylase and cortisol may be suggested as important parameters of oral health.