In this study, a total of 84 milk samples (60 nonbranded and 24 branded) were analysed for occurrence of oxytocin (OT) residues by ELISA. OT level was detected in the range of 39.29-456.55 pg mL À1 with mean value of 138.53 ng L À1 . In branded milk samples, OT residues were minimal as compared to nonbranded milk samples. Nonbranded milk samples had higher prevalence of OT residues; 16.67% had OT level in the range of ≤500 ng L À1 . The average daily intake of OT was found 0.087 lg day À1 per person. It was concluded that the OT concentration was relatively higher in samples obtained from dairy farm, dairy shop and milk man than that in ultra-high temperature (UHT) and powder milk samples. In addition, there are no reports subjected to standardising a permissible OT level in dairy milk category, although OT is considered detrimental to both human and animal life.