Mustafa et al.: Relationship between Tumor Necrosis Factor Alpha-308 and Interleukin-1beta C+3953T Polymorphisms with Male FertilityMale infertility is influenced by psychological, biological and environmental factors. Nowadays, the morphological change of genes and biological molecules are the focus of attention. Therefore, this study was conducted with the aim of investigating the relationship between tumor necrosis factor alpha-308 and interleukin-1beta C+3953T polymorphisms with male fertility in Erbil province. This study was conducted between 2020 and 2021 on 270 men who referred to the infertility center in Erbil, Iraq, with infertility problems. The sampling was purposeful and the samples were divided into three groups of 90 people with the characteristics of the first group without fertility problems, the second group with primary infertility and the third group with secondary infertility. Blood samples were taken from each sample and the samples were sent to the laboratory for deoxyribonucleic acid extraction and allele analysis. A polymerase chain reaction machine was used for this purpose. Statistical package for the social sciences version 23 software and descriptive statistical tests, chi-square, odds ratio and conditional regression were used to analyze the obtained data. The results of tumor necrosis factor alpha-308 genotype showed the frequency of alleles in three groups of men, it can be concluded that there is a significant difference between the three groups of men in terms of mutation frequency and fertility (p≤0.001). The results of interleukin-1beta C+3953T genotype have shown that there is a significant difference in mutation frequency and finally fertility in three groups of men (p≤0.001). The tumor necrosis factor alpha-308 genotype GA and GG alleles have a significant relationship with male fertility. In genotype interleukin-1beta C+3953T, CT and CC alleles have a significant relationship with male fertility. The results of this study showed that tumor necrosis factor alpha-308 genotype and interleukin-1beta C+3953T genotype were identified as important risk factors in male infertility.