This study was designed to investigate the prevailing status of Trichomonas vaginalis, a protozoan parasite causing urogenital tract infections in human beings, in Quetta, Balochistan, Pakistan. As no documented study of this kind has been conducted so far, it was designed to obtain the overall prevalence of T.vaginalis infection. Through conventional wet mount technique, its morphological identification by staining techniques and with PCR confirmation. In addition to investigation of age-wise and gender-wise prevalence of T.v, its correlation with reproductive disorders was also investigated through a questionnaire proforma while taking urine samples. For this purpose six hundred (600) urine samples (300 samples from each gender) were collected from the public sector hospitals located in Quetta city. The study was designed in three age groups as G1: 21-30, G2: 31-40, and G3: 41-50 years of each gender. A total of four samples were found positive microscopically only in female patients, whereas three of these positive samples were confirmed as T. vaginalis by PCR using BTUB9/2 set of primers. The fourth positive sample might be the specie other than T.vaginalis. It is concluded that the overall prevalence of T.v amongst females was observed as 0.1%, while the samples from males were found negative for T.v. so the overall percentage in total samples found as 0.5%.

Thyroid gland is an endocrine gland that reproduces hormones that regulates the body metabolic rate and other body functions. Hyperthyroidism is the dysfunction of thyroid gland which interferes during 2nd and 3rd trimester of pregnancies inducing pre-eclamptic disorder in pregnant women. These disorders may cause deaths as well as morbidity losses in mothers and fetus. These conditions are observed due to imbalance production of T3, T4 and TSH. Hyperthyroidism is highly associated with the onset of pre-eclampsia. Pre-eclampsia is detected by elevated levels of proteinuria as well as systolic and diastolic blood pressures. This study was conceded to discover the influence of hyperthyroidism on pre-eclamptic women of Balochistan. For the purpose, one hundred one (101) pregnant women of 15-50 years of age were selected for this study for comprehensive history, physical and medical examination according to the inclusion and exclusion criteria. Patients of different ethnic groups were examined at Out Patient Department (OPD) in Centre for Nuclear Medicine and Radio therapy (CENAR), Sandeman Provincial Hospital and Bolan Medical Complex hospital (BMC), Quetta. The study focuses on the identification of factors associated with hyperthyroidism and pre-eclampsia and to create awareness regarding this important health issue during pregnancy which may lead to eclamptic seizures during onset of full term labor and are found to be fatal for women and neonate during pregnancy. The current study revealed that out of 101 hyperthyroid patients 61.4% and 20.8% patients belong to Pashtoon and Baloch ethnic groups respectively. It was also found that 44 out of 101 patients lie in age group of 26-35 years. It was observed that highly educated, socioeconomic sound women developed less Pre-eclampsia. Pre-eclampsia was found to be in a significant numbers (33), in hyperthyroid patients, with highest frequency of Pedal edema (36.6%).