ABSTRACT:The aim of the present study was to identify the prevalence of non-viral etiological agents, their geographic, climatic and occupational risk factors associated with microbial keratitis in patients of Kannauj district of Uttar Pradesh, India. Microbial keratitis is a leading cause of monocular blindness world-wide. This study was conducted to find out the epidemiological factors associated with this disease in our set up. METHOD: A prospective analysis of 250 clinically diagnosed cases coming to OPD of Govt. Medical College, Kannauj were examined over a period of one year, from 1 st January 2013 to 31 st December 2013. Age, Sex, Occupation, Agents leading to the ulcer were some of the parameters included in our study. Corneal scrapes were collected and subjected to microscopy, culture and sensitivity. RESULTS: In 250 patients of clinically diagnosed microbial keratitis, 153(61.2%) were Males and 97(38.8%) Females. Most common Age group was 41-60 yrs. (40.8%) followed by 21-40 yrs. (39.2%). Most common cause of Microbial keratitis was Ocular Trauma. Among the microbial keratitis fungal ulcer were more frequent due to trauma by Organic Vegetative matter and as Kannauj is rural area, so Farmers were mostly affected (54%). CONCLUSION: Microbial keratitis is mostly seen in Farmers in this part of India. Males are affected more than Females. Aspergillus and staphylococcus aureus were the most common causative agents. Increased prevalence of Microbial keratitis was seen during harvest and post-harvest seasons.