Objective: To determine the comparison between the capillary glucose and venous plasma glucose in type II diabetic patients. Study Design: Cross Sectional Observational study. Setting: Al–Tibri Medical College and Hospital Karachi. Period: 1st January 2020 to 30th April 2020. Material & Methods: A total of 86 diabetic patients from the out-patient department with non-probability consecutive sampling were selected. Subjects were diagnosed cases of type II diabetes based on the screening recommendation by the American Diabetes Association between 35-75 years of age of either gender included in this study. Patients with type I diabetes, pregnant females and hematocrit of more than 55% or less than 35%, and patients taking medications like Acetaminophen, and Ascorbic acid were excluded from the study. Each participant was measured for the capillary blood glucose levels by using Accu Chek, Easy max and On-call EZII, using strips by Glucose Oxidase Method. A venous sample was collected within 5 minutes of finger-prick test in a sodium fluoride tube. A Statistical Package for Social Sciences version 22 was used for data analysis. Results: Out of 86 patients in the study, 48(55.8%) were females and 38((44.2%) were males with a mean age of 47.16±9.916. The mean blood sugars by venous plasma glucose were 249.58±110.75 mg/dl, by Accu Chek 250.47±117.071 mg/dl, by EasyMax 259.26±119.751 mg/dl, and by On-call EZII 249.33±124.380 mg/dl which shows statistically significant correlation with a p value of <0.001. The glucometers accuracy was determined by using Error grid analysis showed a strong positive correlation between the Accu Chek R2= 0.917, EasyMax R2= 0.917, and Oncall glucometers R2= 0.868 with venous plasma glucose with a statistically significant (p <0.001). Conclusion: This study shows a strong positive correlation between capillary and venous glucose measurements.