A world trade in agricultural commodities has contributed significantly to the discussion about potential hazards involved and has increased in particular the awareness of mycotoxins. Safety awareness in food and feed production has also risen due to the simple fact that methods for testing residues and undesirable substances have become noticeably more sophisticated and more reliable at all points of the supply chain. A 3-year survey program was initiated as decided in the 66 th meeting of the Animal Feeds Sectional Committee held at Standards Development Centre, Karachi, Pakistan in order to evaluate the incidence of aflatoxin B1 in poultry feed and feed raw materials in some of the major poultry production areas. A total of 1021 analyses were performed on 639, 92, 77, 86 and 127 samples sourced from North, South, West, East and Central areas, respectively. Overall 61percent of all aflatoxin B1 tests conducted on samples received from all selected survey areas tested positive. The percentages of positive evidenced in North, South, West, East and Central areas were 63, 49, 56, 53 and 69, respectively. Mean levels of aflatoxin B1 in feed ingredients and finished feeds (except layer grower, broiler starter, broiler finisher in mash forms and layer feed in crumb forms) were noted to be higher than safe limit of 20 µg/kg. This is the first comprehensive report on the determination of aflatoxin B1 in poultry feed raw material and finished feed from Punjab, Pakistan.

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