Cryopreservation has become a central technology in many areas of clinical medicine, biotechnology, and species conservation within both plant and animal biology. Cryoprotective agents (CPAs) invariably play key roles in allowing cells to be processed for storage at deep cryogenic temperatures and to be recovered with high levels of appropriate functionality. As such, these CPA solutes possess a wide range of metabolic and biophysical effects that are both necessary for their modes of action, and potentially complicating for cell biological function. Early successes with cryopreservation were achieved by empirical methodology for choosing and applying CPAs. In recent decades, it has been possible to assemble objective information about CPA modes of action and to optimize their application to living systems, but there still remain significant gaps in our understanding. This review sets out the current status on the biological and chemical knowledge surrounding CPAs, and the conflicting effects of protection versus toxicity resulting from the use of these solutes, which are often required in molar concentrations, far exceeding levels found in normal metabolism. The biophysical properties of CPAs that allow them to facilitate different approaches to cryogenic storage, including vitrification, are highlighted. The topics are discussed with reference to the historical background of applying CPAs, and the relevance of cryoprotective solutes in natural freeze tolerant organisms. Improved cryopreservation success will be an essential step in many future areas such as regenerative medicine, seed banking, or stem cell technology. To achieve this, we will need to further improve our understanding of cryobiology, where better and safer CPAs will be key requirements.

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Electronic medical records can help people prevent diseases, improve cure rates, provide a significant basis for medical institutions and pharmaceutical companies, and provide legal evidence for medical negligence and medical disputes. However, the integrity and security problems of electronic medical data still intractable. In this paper, based on the ciphertext policy attribute-based encryption system and IPFS storage environment, combined with blockchain technology, we constructed an attribute-based encryption scheme for secure storage and efficient sharing of electronic medical records in IPFS storage environment. Our scheme is based on ciphertext policy attribute encryption, which effectively controls the access of electronic medical data without affecting efficient retrieval. Meanwhile, we store the encrypted electronic medical data in the decentralized InterPlanetary File System (IPFS), which not only ensures the security of the storage platform but also solves the problem of the single point of failure. Besides, we leverage the non-tamperable and traceable nature of blockchain technology to achieve secure storage and search for medical data. The security proof shows that our scheme achieves selective security for the choose keyword attacks. Performance analysis and real data set simulation experiments shows that our scheme is efficient and feasible.

With the improvement of living standard, people begin to pay more attention to food safety and product quality. Therefore, for consumers, it is necessary to establish a reliable system that can trace the source of products. However, most existing traceability systems tend to lack transparency, data is primarily stored within the enterprise, and the cost of tampering with data is very low. Besides, the supply chain nodes are easy to evade responsibility when product safety or quality issues arise under the traditional centralized management model, and it is difficult to trace the root of issues. The development of blockchain technology provides us with new ideas for realizing the traceability of products in supply chain scenarios. Due to its characteristics of decentralization, transparency, and immutability, blockchain can be effectively used to alleviate the above problems. In this paper, we propose a product traceability system based on blockchain technology, in which all product transferring histories are perpetually recorded in a distributed ledger by using smart contracts and a chain is formed that can trace back to the source of the products. In particular, we design an event response mechanism to verify the identities of both parties of the transaction, so that the validity of the transaction can be guaranteed. And all events are permanently stored in the form of logs as a basis for handling disputes and tracking responsible entities. Furthermore, a system prototype is constructed based on the testing framework of Truffle. The contract code is deployed on a test network TestRpc that runs in local memory, and a decentralized web page interface is implemented based on the prototype. Finally, the system security analysis and experimental results show that our solution is feasible.

The aim of this study was to determine how different membrane-permeable and -impermeable cryoprotective agents modulate tolerance of stallion sperm to osmotic stress and stabilize membranes during cryopreservation. Special emphasis was on hydroxyl ethylene starch (HES), which exposes cells to minimal osmotic stress due to its large molecular weight. Percentages of motile sperm post-thaw were found to be similar when glycerol, sucrose, and HES were used at their optimal concentrations. Percentages of plasma membrane intact sperm after return to isotonic medium were highest for HES. Fourier transform infrared spectroscopy studies were carried out to study subzero membrane phase and permeability behavior. Cryoprotectants were shown to decrease the initial rate of membrane dehydration during freezing, decrease the activation energy for water transport, and increase the total extent of freezing-induced dehydration. Freezing studies with liposomes as a model system showed that only the membrane-permeable cryoprotective agents glycerol and ethylene glycol protected membranes against leakage, whereas egg yolk, sucrose, and HES did not. Differential scanning calorimetry studies showed that sucrose and HES raise the glass transition temperature of the freezing extender and the difference in heat capacity associated with the glass transition. This indicates that these compounds enable formation of a stable glassy matrix at higher subzero temperatures. Sperm cryosurvival rates can be increased by combining different cryoprotectants with different protective functions; membrane permeable cryoprotective agents stabilize membranes and modulate the rate of cellular dehydration, whereas di- and polysaccharides increase the glass transition temperature and facilitate storage and handling at higher subzero temperatures.