Noninvasive flexible electrochemical sensors for body fluid analysis are subject to strict requirements for sensitivity and detection limit. In this work, a three-dimensional (3D) flexible biosensor based on reduced graphene oxide (rGO) nanocomposites was fabricated for detecting glucose in sweat. Graphene oxide (GO) nanosheets were first assembled onto the surface of silicon dioxide (SiO 2 ) nanospheres by electrostatic interaction and in situ reduced by tannic acid (TA) to obtain the electroactive rGO/SiO 2 nanocomposites. The 3D flexible biosensors were then prepared by successive dropping of the rGO/SiO 2 nanocomposites, glucose oxidase (GOx), and Nafion (NF) solution on the conductive substrate (C-PDMS) composed of a carbon nanotube film and polydimethylsiloxane. Herein, the 3D electroactive structure was designed to maximize the specific surface area of the biosensor, significantly increasing the sensing and recognition sites. The NF/GOx/rGO/SiO 2 /C-PDMS biosensor exhibited good linear detection of glucose in the range of 0.1−9 mM with a sensitivity and lower detection limit of 60.8 μA mM −1 cm −2 and 3.7 μM, respectively, which is superior to existing sensors. Moreover, the biosensor performed well in the detection of glucose from sweat samples and is expected to be used in clinical analysis, offering a new design strategy for point-of-care testing of target molecules.

Objective: To observe the clinical effect of Dahuang Lingxian Decoction in preventing postoperative recurrence of cholelithiasis. Methods: 105 patients of cholelithiasis were randomly divided into 3 groups, receiving clinical treatment and 12 months observation. Respectively, 36 cases in the group of Dahuang Lingxian Decoction were treated with modified Dahuang Lingxian Decoction. 33 cases in blank group did not take any medicine of relieving gallbladder and discharging stone except the routine therapy in preoperational period; 36 cases in Xiaoyan Lidan group administered tablets of Xiaoyan Lidan. Results: After 3 treatment courses, it showed a total effective rate of 97.22% in Dahuang Lingxian Decoction group, 81.8% in blank group, and 83.3% in Xiaoyan Lidan group. Analyzed by statistics, the curative effects between 3 groups had obvious differences (P<0.05), and Dahuang Lingxian Decoction group was significantly superior to blank group and Xiaoyan Lidan group(P<0.05).Conclusions: Dahuang Lingxian Decoction had preferable efficacy on Cholelithiasis and it is worthy of further promotion in clinical application. Keywords: Dahuang lingxian decoction, Postoperative recurrence of cholelithiasis, Clinical researchCholelithiasis is one of the common and frequently-occurring surgical diseases of bile duct (Huang, Zhiqiang, 1998). Treatment of cholelithiasis is very thorny and postoperative recurrence is baffling question in surgical treatment. Reducing recurrence has been always an important project of clinical research.Having the functions of smoothing liver and relieving gallbladder and discharging stone, the decoction is an effective prescription developed from experiences. Through previous clinical researches and animal experiment, it indicated that it could significantly reduce postoperative recurrence and residual stone rate of cholelithiasis and prevent from suffering pigment stone. In order to study it further, we selected cholelithiasis patients as the research objects and administrate red them modified decoction after surgery. The decoction was properly adjusted according to patients' TCM differentiation.