Objective: The study aimed to have a prospective study of serum folate concentration and hemoglobin (Hb%) in cervical carcinoma patients. Methods: The study was conducted on women suffering from carcinoma cervix. The research was conducted in the Gynecological Outpatient Department (OPD) of the Oncology Unit in Mahavir Cancer Sansthan Patna, Bihar. The study period was from May 2016 to April 2017. The sample was collected after face to face interview by Figo staging and the sample was taken regularly. As a result strong association of Hb% and carcinoma cervix was found with the mean value of lower than 11.12, which is lower than the normal range of Hb%. As per the further estimation of folic acid, the level of cervical cancer is low. Results: The data were estimated with help of tools such as SPSS 16.0 and PRISMA 5.0. The Hb% the mean value of patients was estimated to be lower than 11.12, which is not under the normal range of Hb%, that is, nearly 12.5 mg/dl. The low mean value shows less Hb% content which leads to anemia in normal beings as well as in patients with cervical cancer. A strong association of Hb% which affected to patients in carcinoma cervix can be seen in the graph that the mean value of patients low 11.12±0.20 which not comes under normal range of Hb%, that is, 12.5 mg/dl. The R square value is 0.9556. Conclusion: As per our study, we have estimated that the folic acid level in cervical cancer is low. The -normal range of folic acid level in plasma serum is 2.2–20 ug/ml. The mean of 20 control cases, new cervical cancer patients, on treating patients, and follow-up patients is 11.08, 2.206, 3.554, and 2.783, respectively. The control 11.08 shows the better level in plasma serum level comes under a normal range of folic acid level.