The fall armyworm (FAW, Spodoptera frugiperda), has rapidly spread from American continent to many other areas and caused serious damage to local agriculture. In order to explore a more environment-friendly and effective control strategy against this pest, we developed a dual-effect RNAi system based on baculoviruses infection and gene silencing. V-ATPase subunits A and B of FAW (SfvATPaseA and SfvATPaseB) were cloned for the construction of recombinant baculoviruses (rBviruses) of Bacmid-dsSfvATPaseA and Bacmid-dsSfvATPaseB with the capacity of producing small interfering RNA. The efficacy of baculovirus-mediated RNAi was evaluated by injection delivery. The results showed that the relative expression levels of SfvATPaseA and SfvATPaseB were significantly suppressed by constructed rBviruses with reduction rates of 73.85% and 69.82%. The larval inhibition rates of the body weight and length resulting from RNAi were more than 82% and 40%, respectively. More importantly, these two rBviruses greatly accelerated the larval death of FAW with a total mortality using two days less than that caused by bacmid control. After ten serial passages, both rBviruses exhibited excellent genetic stabilities. These results suggest that the creation of dual-effect biopesticides with both activities of viral infection and gene silencing is feasible and prospective.
The fall armyworm (FAW, Spodoptera frugiperda), has rapidly spread from American continent to many other areas and caused serious damage to local agriculture. In order to explore a more environmentfriendly and effective control strategy against this pest, we developed a dual-effect RNAi system based on baculoviruses infection and gene silencing. V-ATPase subunits A and B of FAW (SfvATPaseA and SfvATPaseB) were cloned for the construction of recombinant baculoviruses (rBviruses) of Bacmid-dsSfvATPaseA and Bacmid-dsSfvATPaseB with the capacity of producing small interfering RNA. The e cacy of baculovirus-mediated RNAi was evaluated by injection delivery. The results showed that the relative expression levels of SfvATPaseA and SfvATPaseB were signi cantly suppressed by constructed rBviruses with reduction rates of 73.85% and 69.82%. The larval inhibition rates of the body weight and length resulting from RNAi were more than 82% and 40%, respectively. More importantly, these two rBviruses greatly accelerated the larval death of FAW with a total mortality using two days less than that caused by bacmid control. After ten serial passages, both rBviruses exhibited excellent genetic stabilities. These results suggest that the creation of dual-effect biopesticides with both activities of viral infection and gene silencing is feasible and prospective. Key MessageThe fall armyworm is voracious and destructive and has caused serious losses to agricultural production.RNAi-based control is an effective and environment-friendly strategy to manage this pest.A dual-effect RNAi system based on baculovirus infection and gene silencing was developed to interfere with the V-ATPase of FAW.Baculovirus-mediated silence of SfvATPaseA and SfvATPaseB resulted in very signi cant growth inhibition and mortality of FAW through injection of dsRNA-producing rBviruses.
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