The micronutrient required in minute quantity and their deficiency leads to diminished growth and yield of crops. The field experiment was carried out at College Farm, Rajendranagar during rabi 2015-16 with factorial randomized block design with two factors sorghum genotypes and zinc levels. Findings revealed that varieties and fertilization spray had significant on zinc concentration and uptake by grain and stover of sorghum. Variety CSV -31 (V2) and soil application of zinc sulphate @ 50 kg ha -1 + foliar spray of ZnSO4 @ 0.2% at knee high stage and at flowering (F5) gave significantly highest zinc concentration and uptake by grain and stover in comparison to their respective treatments.