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SUMMARYSpecific polyclonal antisera to human interferon-~l (HulFN-~I), human interferon-~2 (HulFN-~2) and human lymphoblastoid interferon (HulFN-ctLy Namalwa) have been raised in rabbits and sheep. The antisera raised against HulFN-~I and HulFN-~2 strongly neutralized the antiviral activity of their homologous IFN-~ subtypes, but were less active against the heterologous IFN-~ subtypes and preparations containing mixtures of IFN-~ subtypes, e.g. human leukocyte interferon (HulFN-aLe). Antisera raised against HulFN-aLy Namalwa strongly neutralized the antiviral activity of all IFN-~-containing preparations and showed weak cross-reactivity with human interferon-fl (HulFN-fl). Neither anti-HulFN-~l nor anti-HulFN-~2 could be demonstrated to neutralize the antiviral activity of HulFN-fl. A number of monoclonal antibodies to HulFN-a2 have been prepared and these were found to neutralize HulFN-~2 antiviral activity to varying degrees, but not to neutralize the heterologous subtype HulFN-~ 1, preparations containing mixtures of IFN-~ subtypes or HulFN-fl.

An immunoprecipitation technique, which uses radiolabeled human gamma interferon (HuIFN-gamma) and a sheep anti-mouse F(ab')2 serum, is described for the rapid detection of mouse monoclonal antibodies to IFN-gamma in hybridoma culture supernatants. This technique which can be performed in 4 h detects both neutralizing and nonneutralizing antibodies to IFN-gamma. Certain monoclonal antibodies prepared against natural (glycosylated) IFN-gamma which neutralized the antiviral activity of natural IFN-gamma bound strongly to rDNA (unglycosylated) IFN-gamma derived from E. coli, but failed to neutralize its antiviral activity.

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