Introduction: The effusion fluid analysis plays an important role in neoplastic conditions. The present study was undertaken to highlight the frequency of malignant cells in various body fluid. Materials and Methods: This observational study was performed for the period of one and half year. A total of 250 various type of body fluid samples received during the study period in the

Background:Oral lesions can closely resemble one another and hence it is important for the clinicians to be able to recognize the various conditions and if possible obtain a biopsy for a confirmatory pathological diagnosis. Aim:To study the type of common benign lesions of the oral mucosa and to evaluate the concordance of clinical and histopathological diagnosis. Materials And Methods:A retrospective study was carried out in a tertiary care hospital in South Bangalore during the period of two years from June 2016 to May 2018. The study included 50 cases of benign lesions of the oral cavity. The following parameters were analyzed age and sex distribution of the lesion, site of the lesion, clinical presentation and histopathological diagnosis. Data collected were analyzed. Results:Among the 50 cases, the age ranged from 6 to 70 years with a mean of 30.3 years. Most of the patients were females (68%) in their 3rd decade. The sites of involvement of various lesions were labial mucosa with lower lip more commoner than upper lip, followed by tongue, buccal mucosa and maxillary alveolus. The various clinical diagnosis of the lesions were mucus retention cyst, papilloma and pyogenic granuloma. Excision biopsy of these lesions confirmed most cases of mucus retention cyst, pyogenic granuloma and few cases of papilloma on histopathological examination. Rest of the cases were extravastion cyst, fibromyxoma, actinomycosis, lobular capillary hemangioma, hamartoma, angiolymphoid hyperplasia with eosinophils and keratosis without atypia. Conclusion:Benign lesions of the oral cavity mimic each other. Histopathological typing of the lesions is mandatory to rule out malignancy and for a definitive and a confirmatory diagnosis.

Abstract-This study presents bacteriocin (antimicrobial compound), produced from Bacillus subtilis isolated from dairy products. The strains from unpasteurized milk that showed the largest zone of growth inhibition against the indicator strain, Micrococcus luteus was selected for the study. The selected strain was identified as Bacillus subtilis based on its morphological, physiological and biochemical characteristics. The antibiotic susceptibility tests for B.subtilis against various antibiotics demonstrated high sensitivity to Tetracycline, Moderate and less sensitivity towards Streptomycin and Chloramphenicol respectively while being resistant to penicillin. Culture filtrate containing bacteriocin showed the nature of its thermostability (up to 100°C), expressed a pH tolerance (2.0-10.0), and emerged resistance towards trypsin and pepsin. Partial purification of bacteriocin was performed using ammonium sulphate precipitation and the dissolved crude antimicrobial material was then purified by passing into a column of Silica gel. In SDS-PAGE analysis, purified bacteriocin isolated from Bacillus subtilis showed the molecular weight to be approximately 3.4 KDa. The isolate Bacteriocin was tested finally for antibacterial activity against E. coli, associated with food borne illnesses. The zone of inhibition clearly proved that bacteriocin has inhibitory effect on the food pathogen E.coli, thus confirming its bactericidal action in food.