Objective: The study was aimed to evaluate the DNA protective effects of ethanolic extracts of Tinospora cordifolia stem on Swiss albino mice lymphocytes against the sodium arsenite-induced genotoxicity.
Methods: In this experiment, 25 animals of nearly the same age were equally divided. Group 1 (control) fed on tap water while rest four (2, 3, 4, and 5) groups were treated with 4 mg/kg body weight, per orally sodium arsenite for 12 weeks. Sodium arsenite pretreated groups (4 and 5) were followed by administration of 80 mg/kg body weight, per orally T. cordifolia extracts for the next 12 weeks. Animals were sacrificed at the 8th week and 12th week, respectively, for the assessment of sodium arsenite-induced genotoxicity and comparative genoprotective effects of experimental plant extracts. The extent of DNA migration is directly proportional to the magnitude of DNA damage. Percentage tail DNA content and olive tail moment parameters were used in the comet to relate DNA damage.
Results: The findings suggested that the ethanolic stem extracts of T. cordifolia significantly inhibited the sodium arsenite-induced oxidative genotoxicity. The phytoconstituents of T. cordifolia shown to retard genetic damage associated with arsenic exposure.
Conclusion: T. cordifolia may be used as a preventive herbal preparation against chemical or arsenical toxicity.
The present experiment was carried out in cauliflower to study the effect of boron and zinc on growth and yield in the early cauliflower having combinations of four levels 0, 1, 2, 3 kg ha-1 of boron and four levels 0, 2.5, 5.0, 7.5 kg ha-1 of zinc in a factorial randomized block design with three replications during the year 2016 and 2017 respectively. The results of the experiment with applied boron and zinc had significant effect on growth and yield of cauliflower. The highest curd depth (8.88 cm) and curd equatorial diameter (16.45 cm) was found with applied boron (2 kg ha-1) and zinc (2.5 kg ha-1) respectively. While as, maximum net curd weight (410g) and marketable curd weight (695g) was found with sprayed boron and zinc (1 kg ha-1 + 2.5 kg ha-1) respectively., Maximum harvest index (62.8 %) and yield (113.89 q ha-1) was found when applied with boron (2 kg ha-1) and (2 kg ha-1+ 2.5 kg ha-1) respectively. Maximum curd compactness (34.34) and dry matter compactness (14.48) were found with the application of boron (1 kg ha-1) and zinc (2 kg ha-1+2.5 kg ha-1) respectively. Overall findings indicated that with the increase in concentration of boron and zinc the vegetative growth and the reproductive stage of cauliflower increases respectively.
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