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Bakground: Kaempferia parviflora (zingiberaceae family) rhizomes are used for various pharmacological applications. The present study is aimed for putative identification of phyto-constituents in the chloroform extract of the rhizome of Kaempferia parviflora and to correlate with bioactivities like antioxidant and anti-microbial/fungal activities. Methods: Rhizome extract was prepared using Soxhlet extractor and undergone the phytoconstituents analysis of it by gas chromatography-mass spectrometry (GC-MS). Results: Identified twenty-five compounds, out of which 4H-1-Benzopyran-4-0ne,5,7-dimethoxy-2-phenyl and Hydroxyurea showed the maximum and minimum relative abundance of 76.4% and 0.002% respectively. By agar well diffusion method, the extract (25 μg/ml) exhibited highest antibacterial potential against Micrococcus luteus (MTCC 106) (gram-positive bacteria) and anti-fungal potential against plant pathogen Rhizoctonia solani (MTCC 4633). The extract also showed the potent scavenging activities of 2,2-diphenyl-1-picryl-hydrazine (DPPH) and 2,2′-Azino-bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) radicals with IC 50 values of 3.0 ± 0.1 mg/ml and 19.4 ± 1.2 µg/ml respectively. Subsequently, lipid peroxidation assay was also used for estimation of antioxidant activity of this extract. The extract (20 µg/ml) inhibited lipid peroxidation by 80.0 ± 4.5%. Conclusion: The outcomes of present study provide scope for isolation of bioactive compounds from Kaempferia parviflora for medicinal application.