No abstract

In this work, sensitive method was used for the first time by joining cloud point extraction with solvation system (CPE-SS) using aspirin as the organic reagent to form cloud point layer (CPL). It had a wavelength for final absorbance under λ max = 293 nm. Different kinds and concentrations of salting out with different extraction efficiencies were used to enhance the extraction efficiency. KNO 3 as a salting out under 0.1 M showed maximum extraction efficiency in the presence of 40 µg of Cu(II). The applications of extraction in this method had required the existence of 0.4 mL of Triton X-100 and heating under 85 °C for 20 minutes to form CPL quantitatively. The effect of different surfactants and various organic reagents to investigate the impact of interferences as well as spectrophotometric determination were also investigated in this study. Chicken, breast, cow meat (beef), cucumber, drainage fish, garden cress and lettuce showed low limit of detection (LOD) 0.018 ppm, limit of quantity (LOQ) 0.060 ppm and RSD% of 0.028 in 2 ppm.

Spectrophotometric  study for ion pair complex of Ni(II) ion extracted into cloud point extraction larger appear wave length of maximum absorbance was λ=660 nm, for extracted Ni(II) ion as ion pair association ,used laboratory synthesized azo derivative 2-(4-hydroxy phenyl azo)-4-Benzen naphthol(HPAB).The study about Determination the optimum condition for extraction and  determination was pH  =10 in presence 100 µg Ni(II) in 10 ml aqueous solution ,0.6 mL of Triton x-114 and temperature of heating was 70 c° at heating time equal to 30 minute, stoichiometry shows the ion pair complex extracted was 1:1 (Ni-HPAB) +2;2Cl_, in addition to electrolyte effect study as well as spectrophotometric determination of Ni(II) in different samples .