BackgroundBecause corneal infiltrative events (CIEs) may result from bacterial components on contact lenses, which can come from contaminated lens cases, we evaluated the biocidal efficacy of five multipurpose solutions against Gram‐negative commonly isolated and CIE‐associated organisms.MethodsOf the multipurpose solutions tested, one contained polyhexamethylene biguanide (PHMB)/polyquaternium‐1 (PQ‐1; Bausch & Lomb Incorporated: Biotrue), one contained alexidine dihydrochloride (alexidine)/PQ‐1 (AMO: RevitaLens OcuTec) and three contained PQ‐1/myristamidopropyl dimethylamine (MAPD; Alcon: Opti‐Free PureMoist, PQ‐1/MAPD‐1; Opti‐Free RepleniSH, PQ‐1/MAPD‐2; Opti‐Free Express, PQ‐1/MAPD‐3). Challenge organisms were CIE‐associated Achromobacter xylosoxidans, Delftia acidovorans and Stenotrophomonas maltophilia at manufacturer‐recommended durations (stand‐alone), in lens cases without lenses (up to seven days) and in lens cases with etafilcon A lenses (up to 30 days).ResultsIn stand‐alone testing against CIE‐associated organisms, PHMB/PQ‐1 and alexidine/PQ‐1 were significantly superior versus MAPD‐based multipurpose solutions against A. xylosoxidans (all p ≤ 0.01), D. acidovorans (all p ≤ 0.001) and S. maltophilia (all p ≤ 0.05). In lens cases, PHMB/PQ‐1 and alexidine/PQ‐1 achieved greater than 3‐log reductions against all challenge organisms at all times evaluated. PQ‐1/MAPD‐1 achieved a greater than 3‐log reduction against D. acidovorans at 24 hours; PQ‐1/MAPD‐1 and PQ‐1/MAPD‐3 achieved greater than 3‐log reductions at seven days against all organisms. In lens cases with lenses, PHMB/PQ‐1 and alexidine/PQ‐1 achieved greater than 3‐log reductions against all organisms at all times. PQ‐1/MAPD‐1 and PQ‐1/MAPD‐3 achieved greater than 3‐log reductions at seven or more days against all organisms. PQ‐1/MAPD‐2 did not achieve a greater than 3‐log reduction at any time; some regrowth was observed.Conclusions PHMB‐ and alexidine‐based multipurpose solutions demonstrated significantly greater biocidal activity compared with PQ‐1/MAPD‐based agents against Gram‐negative organisms commonly isolated and CIE‐associated pathogens.

PurposeTo determine whether agents which are purportedly capable of inducing encystment of Acanthamoeba can recapitulate the signal when tested in differing formulations.MethodsIn accordance with the International Standard ISO 19045, Acanthamoeba castellanii ATCC 50370 trophozoites were cultured in antibiotic-free axenic medium, treated with test solutions, and encystment rates plus viability were measured via bright field and fluorescent microscopy. Test solutions included phosphate-buffered saline (PBS), borate-buffered saline, biguanide- and hydrogen peroxide (H2O2)-based biocides, propylene glycol (PG) and povidone (POV) ophthalmic demulcents, and one-step H2O2-based contact lens disinfection systems.ResultsOnly PBS solutions with 0.25 ppm polyaminopropyl biguanide (PAPB) and increasing concentrations of PG and POV stimulated A. castellanii encystment in a dose-dependent manner, whereas PBS solutions containing 3% H2O2 and increasing concentrations of PG and POV did not stimulate encystment. Borate-buffered saline and PBS/citrate solutions containing PG also did not stimulate encystment. In addition, no encystment was observed after 24 hours, 7 days, or 14 days of exposures of trophozoites to one-step H2O2 contact lens disinfection products or related solutions.ConclusionThe lack of any encystment observed when trophozoites were treated with existing or new one-step H2O2 contact lens care products, as well as when trophozoites were exposed to various related test solutions, confirms that Acanthamoeba encystment is a complex process which depends upon simultaneous contributions of multiple factors including buffers, biocides, and demulcents.

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