BackgroundMultiplicity and genetic diversity of Plasmodium falciparum infection might play a role in determining the clinical outcome of malaria infection and could be a fair reflection of the disease transmission rate. This study investigated the genetic diversity of P. falciparum and multiplicity of infection in relation to the severity of malaria and age of patients in Gezira State, Sudan.MethodsA cross-sectional health facilities-based survey was conducted in Gezira State, Sudan in January 2012. A total of 140 P. falciparum malaria patients diagnosed with microscopy and confirmed using nested PCR were recruited and classified into uncomplicated malaria and severe malaria states according to the standard WHO criteria. DNA was extracted and MSP1 and MSP2 allelic families were determined using nested PCR.ResultsThe overall multiplicity of infection (MOI) was 2.25 and 2.30 and 2.15 for uncomplicated and severe malaria respectively. There were no statistically significant differences between uncomplicated and severe malaria (SM) patient groups in MOI with regard to MSP1, MSP2 and overall MOI (Mann-Whitney U-test; all P < 0.05). The predominant MSP1 allelic families were MAD20 for uncomplicated malaria and RO33 for severe malaria. The distribution of both FC27 and IC1/3D7 MSP2 allelic families were approximately the same across disease severity. One hundred and eleven P. falciparum isolates (81 %) consisted of multiple genotypes; 71/90 (78.9 %) in uncomplicated malaria and 40/50 (85.1 %) in severe malaria patient groups. Neither MSP1 nor MSP2 allelic families showed association with malaria severity. No statistically significant differences in multiplicity of infection were observed between different age groups.ConclusionIn this study the majority of P. falciparum isolates from uncomplicated and severe malaria patients consisted of multiple genotypes. Further molecular epidemiological studies delineate the link between P. falciparum genotype with the malaria phenotype in different regions are encouraged.

Malaria is a major health problem, with over one third of Background worldwide populations currently at risk. Determining the genetic diversity of plasmodium parasites is essential for assessing the efficacy of antimalarial drugs and for future vaccine development. This study investigated the genetic diversity of merozoite surface protein 2 (MSP2), and multiplicity of P. falciparum infection (MOI) in different geographic regions in Sudan.: A total of 271 patients with uncomplicated malaria were recruited Methods from four ecological sites during malaria transmission season, 2011-2013. P.was confirmed using species specific primers targeting the rDNA falciparum gene. All positive samples were genotyped for the major MSP2 P. falciparum allelic families (IC1/3D7 and FC27 MSP2 allele) using nested PCR. Multiplicity of infection and allele frequencies were determined.: A total of 241 samples (88.9%) were confirmed positive for Results P. . The number of different alleles were 14, 15, 13 and 12 in falciparum MSP2 Khartoum, Gezira, River Nile and Red Sea states, respectively. The 3D7 allelic family was more prevalent in the states of Khartoum, Gezira, River Nile and Red Sea compared to the FC27 allelic family. Multiclonal infections were observed in 25.8% of patients, with a mean multiplicity of infection (MOI) of 1.45. MOIs were highest in the age group over 40, with an average of 2 and 1.68 in Khartoum and Gezira states, respectively, however MOIs in River Nile and Red Sea states were higher in age groups below 18, with an average of 1.37 and 1.33, respectively.: MSP2 allelic genotyping revealed MOI and diversity of the Conclusions Sudanese isolates. The results of our study are expected to P. falciparum influence current and future malaria control strategies, since the MOI predicts development of clinical malaria and subsequent efficacy of antimalarial treatment.

Moringa oleifera grown and used in many countries around the world is a multi-purpose tree with medicinal and nutritional values. This study evaluates the effect of taking moringa oleifera leaves powder on blood sugar levels in humans, and study side effect on kidney (urea, creatinine) and liver enzyme (AST, ALT) after taken leaves powder for a month. The study targeted patients who their blood glucose not lowering by drugs. Blood glucose, urea, creatinine, AST and ALT for all diabetic patients was determined before and after taking 0.5g leaves powder. Results showed blood sugar levels decreased statistically significant (p<0.001) for all diabetic patients, and no statistically significant difference in the mean values of urea, creatinine, (AST) and (ALT) before and after taking leaves powder (p˃0.001). This study concluded that leaves powder have a significant impact on anti-diabetic property for the selected patients, so it's promising in the prevention for risk of diabetes mellitus.