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A 3-yr study utilized 300 Angus-based, spring-born heifers to evaluate postweaning heifer development systems on gain, reproductive performance, and feed efficiency as a pregnant heifer. Heifers were blocked by BW and randomly assigned to graze corn residue (CR), upland range (RANGE), or were fed 1 of 2 diets in a drylot differing in energy levels: high (DLHI) or low (DLLO). Heifers developed on DLHI and DLLO were managed within the drylot for 166 d in yr 1, 150 d in yr 2, and 162 d in yr 3. Heifers developed on RANGE grazed winter range for an equivalent amount of days each yr as the DLHI and DLLO heifers. Heifers assigned to CR grazed for 103 d in yr 1, 84 d in yr 2, and 97 d in yr 3 before being transported to graze winter range for the remainder of the treatment period. All heifers were managed as a single group following the treatment period. Artificial insemination and natural mating were utilized during breeding. Percent of mature BW prior to the breeding season was greater ( = 0.02) for DLHI (67%) compared with RANGE (59%) and CR (58%). Pregnancy rates to AI were not different ( = 0.51) among treatments (59 ± 6%), and final pregnancy rates were also not different (87 ± 4%, = 0.54). A subset of AI-pregnant heifers from each treatment were placed in a Calan gate feeding system. Heifers were allowed a 20-d acclimation period before beginning the 90 d trial at approximately 170 d in gestation. Heifers were offered ad libitum hay; amount offered was recorded daily and orts collected weekly. Initial BW was not different ( = 0.58) among treatments (459 ± 11 kg). Body weight at the end of the trial (497 ± 17 kg) was also not different ( = 0.41). Intake was not different ( = 0.33), either as DMI (10.00 ± 1.07 kg) or residual feed intake (0.018 ± 0.190). There was no difference in ADG ( = 0.36, 0.42 ± 0.23 kg/d) among treatments. Although the total development cost was not different among treatments ( = 0.99), there was a $41 difference ( < 0.01) between the mean of the most expensive diet (DLHI) and the mean of the two least expensive diets (CR and RANGE). Developing heifers to a greater prebreeding BW did not influence subsequent AI or overall pregnancy rates or feed efficiency as a pregnant heifer.

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Follicular progression during peripuberty is affected by diet. Vascular endothelial growth factor A (VEGFA) induces follicle progression in many species; however, there are limited studies to determine if diet may alter the effects of angiogenic VEGFA165-stimulated follicle progression or antiangiogenic VEGFA165b follicle arrest. We hypothesized that diet affects the magnitude of angiogenic and antiangiogenic VEGFA isoform actions on follicular development through diverse signal transduction pathways. To test this hypothesis, beef heifers in our first trial received Stair-Step (restricted and refeeding) or control diets from 8 to 13 months of age. Ovaries were collected to determine follicle stages, measure vascular gene expression and conduct ovarian cortical cultures. Ovarian cortical cultures were treated with phosphate-buffered saline (control), 50 ng/ml VEGFA165, VEGFA165b, or VEGFA165 + VEGFA165b. The Stair-Step heifers had more primordial follicles (P &lt; 0.0001), greater messenger RNA abundance of vascular markers VE-cadherin (P &lt; 0.0001) and NRP-1 (P &lt; 0.0051) than controls at 13 months of age prior to culture. After culture, VEGFA isoforms had similar effects, independent of diet, where VEGFA165 stimulated and VEGFA165b inhibited VEGFA165-stimulated follicle progression from early primary to antral follicle stages. In vitro cultures were treated with VEGFA isoforms and signal transduction array plates were evaluated. VEGFA165 stimulated expression of genes related to cell cycle, cell proliferation, and growth while VEGFA165b inhibited expression of those genes. Thus, VEGFA isoforms can act independently of diet to alter follicle progression or arrest. Furthermore, follicle progression can be stimulated by VEGFA165 and inhibited by VEGFA165b through diverse signal transduction pathways.

No abstract