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C in a 5% CO 2 atmosphere for 2-hour (defined as the optimal incubation time in previous study). Sperm parameters were then analyzed both in fresh samples and after incubation in different media: manual sperm analysis; motility via Sperm Computer Analysis (SCA Ò ); sperm chromatin integrity via Sperm Chromatin Structural Assay (SCSA Ò ); Reactive Oxygen Species level (ROS) by chemiluminescent detection and mitochondrial activity by staining with 3.3 0 -diaminobenzidine (DAB). Data were analyzed using the IBM SPSS software. Analysis of variance (ANOVA) and independent student t-tests were used to evaluating statistical significance (P<0.05).RESULTS: CSCM Ò medium demonstrated a significant increase in postincubation motility (progressive motility ¼ 24.55AE13.97%; total motility ¼ 41.17AE19.06%) compared to pre-incubation parameters (progressive motility ¼ 8.33AE6.82; total motility ¼ 26.61AE18.10%; P<0.025). For all other culture media, no significant differences were observed in seminal parameters.CONCLUSIONS: Asthenozoospermic men may have significant improvement in their semen samples in the andrology lab after a 2-hours incubation in CSCM Ò +HSA Ò medium. This technique has proven to be very attractive because of its efficiency, low cost and ease of reproduction.SUPPORT: Miss Ran ea was a recipient of a Master scholarship from the São Paulo Research Foundation

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