Seven new quinazoline-containing indole alkaloids (1–7) named aspertoryadins A–G, along with nine known ones (8–16), were isolated from the marine-derived fungus Aspergillus sp. HNMF114 from the bivalve mollusk Sanguinolaria chinensis. The structures of the new compounds were elucidated from spectroscopic data, X-ray diffraction analysis, ECD spectra analysis, and ECD calculations. Compound 1 bears an aminosulfonyl group in the structure, which is rarely encountered in natural products. Compounds 6, 7, and 13 exhibited quorum sensing inhibitory activity against Chromobacterium violaceum CV026 with MIC values of 32, 32, and 16 μg/well, respectively.

In the present study, we investigate the mechanism of how p53 induces growth arrest in Rb-defective Saos2 cells that express temperature-sensitive mutant p53 (ts p53). The activation of p53 at a permissive temperature (32.58C) induces the cell cycle arrest at both the G1 and G2 stages. The induction of several p53-responsive genes as well as a small form of p130 (S-p130) was detected upon p53 activation. S-p130 retained the functions as a pocket protein and was dominant over p130 at the protein level after 36 h at 32.58C. A canonical p53 binding site was identified in intron 4 of p130. Furthermore, a novel p53-inducible transcript containing a partial intron 4 sequence downstream of the p53 binding site and exon 5 of p130 was detected by RT -PCR, suggesting S-p130 is induced by p53 at transcriptional level. The results from gel shift assay and immunoprecipitation showed that Sp130 as well as p130 formed complexes with both E2F1 and E2F4 at a permissive temperature. Moreover, the transient expression of E1A (12S) and E2F1 effectively abrogated p53-induced cell cycle arrest. These results strongly suggested that p130 and its truncated form might substitute Rb in mediating p53-induced cell cycle arrest in Rb 7/7 Saos2 cells.

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Abstract. In recent years, MRI studies have resulted in a better understanding of the movement and deformation of the meniscus in the terbinafine connection and provide, information on the morphology of the articular cartilage. In this paper, we will use of computer-aided technique and MRI to reconstruct the shape of the living knee, and divide the mesh and add the material properties to create the three-dimensional nonlinear knee joint finite element model information. Examining the relative sliding and friction in the knee joint, I used the dynamic dominant non-linear finite element technique to simulate the photomechanical properties of the tibial femoral joint under different loads. Apply a pressure, in the 0 degree direction to calculate the change of each structure, including: maximum pressure, average pressure, contact pressure, internal stress. Dimensional Finite Element Model of Knee Joint was Established Based on MRIAll of the data from this study were obtained from the 25-year-old healthy male knee joint. The equipment was selected from GE's MRI Signa product line and equipped with DICOM 3.0 international standard interfaces for image output of DICOM standard format. Simplware Software to Build STL Format ModelWe use image processing software Simpleware-ScanIPV3.1 to complete the entire area of the knee the three-dimensional reconstruction of the structure. Since the whole image needs to be divided into the patella, femur, tibia and their surface of the cartilage and medial and lateral meniscus, need to establish a number of different mask processing, and finally through the Boolean operation to remove the overlap between the different parts of the output data.