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Information technology has developed rapidly in the past decades, in particular the use of service-oriented architectures, which have been widely adopted in Taiwan's healthcare industry. Radio-frequency identification (RFID) is one of the most widely applied new technologies. Using the unified theory of acceptance and use of technology model, this study investigated the effect of perceived security (PS) and privacy concerns (PC) on health professionals' RFID usage behaviour (UB), and the effect of UB on organisational performance (OP). A questionnaire survey was used and 410 valid questionnaires were returned. The results of path analysis revealed that performance expectancy (PE), effort expectancy (EE), social influence, and facilitating conditions (FC) exert a significantly positive influence on UB; PS has a significantly positive effect on PE and PC; and UB exerts a significantly positive influence on OP. Different demographic variables also moderated the effect of PE, EE, and FC on RFID UB.