A predator-prey model with Holling II functional response incorporating a prey refuge with impulse effect is considered in this paper. With the help of the Floquet theory of impulsive differential equations, local stability and global attractivity of the boundary periodic solution of the system are derived, and then sufficient conditions for global asymptotic stability of the boundary periodic solution are obtained. Next, the permanence of the system is proved by constructing a Lyapunov function. Further, by applying the bifurcation theory of impulsive differential equations, conditions under which the system has a positive periodic solution are obtained. Finally, numerical simulations are presented to illustrate the analytical results.