A Gram-negative bacterium, designated S1-65T, was isolated from soil samples collected from a cotton field located in the Xinjiang region of PR China. Results of 16S rRNA gene sequence analysis revealed that strain S1-65T was affiliated to the genus
Steroidobacter
with its closest phylogenetic relatives being ‘Steroidobacter cummioxidans’ 35Y (98.4 %), ‘
Steroidobacter agaridevorans
’ SA29-B (98.3 %) and
Steroidobacter agariperforans
KA5-BT (98.3 %). 16S rRNA-directed phylogenetic analysis showed that strain S1-65T formed a unique phylogenetic subclade next to ‘
S. agaridevorans
’ SA29-B and
S. agariperforans
KA5-BT, suggesting that strain S1-65T should be identified as a member of the genus
Steroidobacter
. Further, substantial differences between the genotypic properties of strain S1-65T and the members of the genus
Steroidobacter
, including average nucleotide identity and digital DNA–DNA hybridization, resolved the taxonomic position of strain S1-65T and suggested its positioning as representing a novel species of the genus
Steroidobacter
. The DNA G+C content of strain S1-65T was 62.5 mol%, based on its draft genome sequence. The predominant respiratory quinone was ubiquinone-8. The main fatty acids were identified as summed feature 3 (C16:1ω6c/C16:1ω7c), C16 : 0 and iso-C15 : 0. In addition, its polar lipid profile was composed of aminophospholipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Here, we propose a novel species of the genus
Steroidobacter
: Steroidobacter gossypii sp. nov. with the type strain S1-65T (=JCM 34287T=CGMCC 1.18736T).
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Genomic molecular signatures determined characterization of
Mycolicibacterium gossypii sp. nov., a fast-growing mycobacterial species isolated from cotton field soilAuthors name:Rui-
Two Gram-positive, aerobic and non-motile actinomycetes, designated S1-96T and N2-109T, were isolated from soils collected from a cotton field. They are described as representing two novel species of genera
Actinophytocola
and
Streptomyces
through a polyphasic approach. Analysis of 16S rRNA gene sequences revealed that strains S1-96T and N2-109T showed highest similarity to
Actinophytocola xinjiangensis
CGMCC 4.4663T (99.10 %) and
Streptomyces iconiensis
BNT558T (98.21 %), respectively. Phylogenetic analyses based on 16S rRNA and core genes confirmed the close relationships of these strains. Genomic analyses further supported the novel taxonomic delimitation of these two species based on digital DNA–DNA hybridization and average nucleotide identity. Strains S1-96T and N2-109T contained MK-9(H4) and MK-9(H6) as the most abundant menaquinone, respectively. High abundances of iso-fatty acids were detected in both strains, which was similar to their close relatives. Physiological and polar lipid analyses also revealed differences between these strains and their phylogenetic neighbours, supporting their taxonomic delimitation as novel species. The names Actinophytocola gossypii sp. nov. (type strain S1-96T=JCM 34412T=CGMCC 4.7707T) and Streptomyces gossypii sp. nov. (type strain N2-109T=JCM 34628T=CGMCC 4.7717T) are proposed.
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