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Introduction: Diabetes mellitus is prevalent all over the world and it is expected to have more than 300 million patients by 2030. Diabetes is affected by various factors such as stress, obesity and others. Objectives:The main objective of this study is to investigate the impact of gender and body mass index on lipid profile of diabetic patients treated in the outpatient clinics at King Hussein Medical Center, Royal Medical Services, Jordan.Methodology: This is a retrospective study and files of patients were reviewed if they were appropriated for this study in terms of the data content compatibility. It should be clearly apparent in files that such important data indicating gender, age, body mass index and lipid profile for each patient exist. A total of 62 files of diabetic patients were selected. A working excel sheet will was created and prepared for data collection. After the entry of all data had been completed, data were analyzed statistically using SPSS version 21. Results:The mean age of study participants was 57±12 years. As shown by body mass index (31.5±21 kg/m 2 ), study participants were obese. Lipid profile was abnormal. Body mass index had impacts on lipid profile of diabetic patients, while gender had no impacts on lipid profile of diabetic patients. Correlation was positively and significantly between age and low-density lipoprotein, and between cholesterol and each of triglyceride, high density lipoprotein, and low-density lipoprotein. Conclusion:The present study showed that obesity as represented by high body mass index has impacts on lipid profile of diabetic patients. On the other hand, gender had no impact on lipid profile of the same patients. Pearson correlation was positively and significantly between cholesterol and triglycerides, high density lipoprotein, and low-density lipoprotein which showed the effect of insulin insufficiency.