This present quantitative study documented the demographic base of 21st century Extension Master Gardener (EMG) volunteers in the United States. As the EMG program approaches its fifth decade and momentum builds for national leadership, collaborative programming, and innovative impact reporting, it is important to understand the characteristics of the current volunteers and their coordinators. A national study of EMG coordinators and volunteers was conducted in Fall 2016. Response was strong, representing 71.4% of state programs and 7498 volunteers. Responding state coordinators are primarily white females, have a mean age of 51.2 years, and have served in their position an average of 7.2 years. Most state coordinators (94.1%) have a graduate degree (master’s or higher). Responding local coordinators are primarily white females, have a mean age of 51.9 years, and have served in their position 7.5 years. Some local coordinators (57.4%) have a graduate degree (master’s or higher). EMG volunteers responding were primarily female, white, educated, retired, and of economic means; have a mean age of 64.8 years; and have served an average of 7.7 years. Four generations [Traditionalist (born 1925–42), Baby Boomer (born 1943–60), GenX (born 1961–81), and GenY (born 1982–2000)] were represented in survey responses. EMG volunteers were 14.5% Traditionalists, 73.2% Baby Boomers, 11.5% GenX, and 0.9% GenY. There were significant differences in the age, age at initial training, years of active service, and service hours reported in 2015 (the prior complete program cycle) among four generations of EMG volunteers. Responses from EMG volunteers and their coordinators represented all six extension programmatic regions established by the EMG National Committee. Significant differences in age, years of service, and number of volunteer service hours reported in 2015 exist among EMG volunteers across extension programmatic regions. The majority of EMG volunteers responding to the survey indicated they volunteered in an urban county (80.5%), whereas 17.2% of respondents served in a suburban county and 2.1% were connected with rural counties. There were no significant differences in the average age, years of service, and number of volunteer service hours reported in 2015 for EMG volunteers in urban, suburban, and rural programs. Historical data and the present study share similar trends within demographics, including age, income, gender, education, and race/ethnicity, yet offer important considerations for future program growth and development.