A new sensitive, rapid and simple spectrofluorimetric method was utilized for the assessment of velpatasvir (VPS) in its bulk form as well as in its combined tablet with sofosbovir (SFV).

Industrial wastewater from soap and oil industries represents a heavy pollution source on their receiving water body. This paper studies a case of pollution control at Tanta Soap and Oil Company, Banha Factory, Egypt. The factory production includes soap, edible oil, and animal fodder. About 4,347 m3/day of industrial wastewater effluent was discharged via gravity sewers to the public sewerage system. Most of the effluent was cooling water because the cooling process in the factory was open circle. In spite of the huge quantity of cooling water being disposed of, disposal of wastewater was violating pertinent legislation. Three procedures were used for controlling the pollution at the Banha Factory. Firstly, all open circuit cooling systems were converted to closed circuit thus reducing the quantity of the discharged wastewater down to 767 m3/day. Secondly, the heavily polluted oil and grease (O&G) wastewater from the refinery unit is treated via two gravity oil separator (GOS) units, dissolved air floatation (DAF), and biological units in order to reduce the high levels of O&G, BOD, COD, and SS to the allowable limits. Thirdly, the heavily polluted waste effluent from the 'red water' saponification unit is treated separately by acidification to convert the emulsified fatty acid to free form in order to be separated through an oil separation unit. The effluent is then passed to liming stage to neutralize excess acidity and precipitate some of the dissolved matters. The mixture is finally clarified and the pH is adjusted to the allowable limits. The effluent wastewater from the three processes is collected and mixed in a final equalization tank for discharging effluent to the public sewerage system. The characteristics of the effluent water are very good with respect to the allowable Egyptian limits for discharging effluent to the public sewerage system.

Two methods, a reversed phase high-performance liquid chromatographic (RP-HPLC) method and a direct ultra-violet spectrophotometric method, were adopted and validated for the quantification of sofosbuvir, which is a new antiviral agent used for treatment of patients with hepatitis C virus (HCV). Validation parameters such as linearity, accuracy, precision, specificity, limits of detection and quantification were determined according to the guidelines of International Conference on Harmonization (ICH)-Q2B. The RP-HPLC method was applied on Hypersil TM ODS C18 column (150×4.6 mm, 5 µm) as a stationary phase. The mobile phase was optimized according to the polarity of the studied drug. It was methanol: acetonitrile (90:10, v:v), pumped using an isocratic mode with flow rate of 1 mL/min and UV detection at 260 nm. The UV spectrophotometric method was performed for the studied drug at 260 nm. The calibration curves were linear in the ranges of 2-60 µg/mL and 5-40 µg/mL for the RP-HPLC and UV spectrophotometric methods, respectively. The proposed methods are accurate, sensitive and precise, so they can be successfully adopted for the reliable determination of sofosbuvir content in its tablet form.