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Self-poisoning with pesticides accounts for approximately one-third of all suicides worldwide. To expedite rescue in the emergency department, it is essential to develop a point-of-care analytical method for rapid identification of ingested pesticides. In this study, five of the most common pesticides ingested by self-poisoning patients in Taiwan were analyzed from oral fluid samples. Pesticide-oral fluid mixtures were applied on a cotton swab and then transferred into methanol. A metallic probe was used to sample the methanol solution for subsequent thermal desorption-electrospray ionization mass spectrometry analysis. Altogether, pesticide sampling, transfer, desorption, ionization, and detection took less than 1 min. The reproducibility of this method (n = 6) was shown in the observed low-relative standard deviation (<7%) in the detection of pesticide in oral fluid. The detection limits of the pesticides in oral fluid obtained from four human subjects by thermal desorption-electrospray ionization mass spectrometry were between 1-10 ppb with relative standard deviation 10.7%. Moreover, in this study, linear responses of five pesticides in oral fluid with concentrations between 1 ppb-1 ppm (R2 between 0.9938 and 0.9988) were observed. As the whole analytical process is extremely short, this technique allows for early non-invasive point-of-care identification of pesticides in the oral fluid of self-poisoning patients in the emergency room, providing important toxicological information for decision-making during critical resuscitation.

Thermoelastic data are combined with an Airy stress function to determine the individual stresses on and near the boundary of a circular hole which is located below a concentrated edge-load in a plate. Coefficients of the stress function are evaluated from the measured temperatures and the local traction-free conditions are satisfied by imposing s rr ¼ t rq ¼ 0 analytically on the edge of the hole. The latter has the advantage of reducing the number of coefficients in the stress function series. The method simultaneously smoothes the measured input data, satisfies the local boundary conditions and evaluates individual stresses on, and in the neighbourhood of, the edge of the hole. Attention is paid to how many coefficients to retain in the stress function series. Although the presence of high stress concentration factors, together with a hole-diameterto-plate-thickness ratio of only two, result in some threedimensional effects, these are relatively small and the agreement between the thermoelastic values, those from recorded strains and FEM-predicted surface stresses is good.

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