The babies born to preeclamptic mothers are more prone for development of prematurity, low birth weight, Intrauterine Growth Retardation (IUGR), sepsis, neutropenia, leucopenia, thrombocytopenia, increased RBC count, nRBC and reticulocyte count. Early haematological screening helps to decrease morbidity, improve growth, development and survival of the baby.
INTRODUCTIONFine Needle Aspiration Cytology (FNAC) is a preliminary diagnostic procedure for wide spectrum of non neoplastic and neoplastic lesions. It is safe faster and cost effective procedure. It is quick, easy to perform and has high grade specificity and sensitivity. It is used as a primary investigation procedure in all the suspected neoplastic conditions before any surgery [1].Routinely various stains like Haematoxylin and Eosin (H&E), Romanowsky and Pap have been used for staining the FNAC smears. Romanowsky stains are routinely used for staining the blood films and air dried cytological smears [2]. The different types of Romanowsky stains available are MGG, Wright Giemsa stain, Leishman stain and Diff Quick. These stains allow better estimation of cell size, nuclear size, cell cytoplasm and identify ground substances by metachromasia [3,4]. MGG is routinely used in cytology for air dried smears in many laboratories. This stain demonstrates the cytoplasmic features but not the nuclear chromatin well and the procedure is time consuming and stain has tendency to precipitate [5]. Leishman stain is a good nuclear stain which is used widely in haematology [6].LG cocktail is a new staining technique with very few studies in the literature describing its applicability in cytology. Few authors have opinioned that LG cocktail stain provides an excellent cytoplasmic and nuclear staining comparable to Pap stain and superior to MGG in both staining quality and diagnostic ability in exfoliative cytology and also has a potential application in the screening programmes [6]. Hence, the study was done to evaluate the quality of staining of LG cocktail in air dried cytology smears and compare the quality of staining of LG cocktail with MGG. MATERIALS AND METHODSThis prospective study was done at a tertiary care centre. The number of cases studied was 100 for duration of two months.Before starting the study ethical clearance was taken from the Institutional Ethical Committee. It included all the patients who were sent to cytology department for FNAC. Informed consent was taken from patient for the FNAC procedure. Cases with inadequate material were excluded from the study. Extra slides were made from the FNAC material. These smears were stained with MGG (Himedia, India) and LG cocktail -Combination of Leishman stain (Chromo span, India) and Giemsa stain (Nice, India) respectively.A total of 200 slides were screened by two pathologists. The slides were stained with MGG by using conventional method. The LG cocktail was prepared by the using the following protocol [2]:1) The unit volume of Giemsa was mixed with equal amount of distilled water to prepare Giemsa working solution (1:1 dilution);2) An equal volume of Leishman's stain was filtered and mixed with an equal volume of the above Giemsa working solution (1:1);LG cocktail staining procedure [2]: The air dried smears were flooded with LG cocktail and left for one minute. An equal volume of buffer/distilled water was added and left for six minutes. The slides were washed ...
Tumours of the colon and rectum are one of the most common malignancies worldwide. In India, its incidence is less compared to the developed countries however,recently it has increased from past few decades due to sedentary lifestyle and more consumption of animal fats with less dietary fibre intake. The liver and lungs are most common sites of metastasis from colorectal carcinoma; however, it occasionally metastasised to atypical sites by making diagnosis difficult. Due to the high frequency of colorectal carcinoma, even infrequent metastatic sites are important to diagnose as early as possible. Hereby, authors present a case of 53- year-old male, with history of carcinoma rectum and treated with Abdominoperineal Resection (APR) with permanent colostomy three years back. Three years after the diagnosis of carcinoma rectum, the patient was diagnosed with metastasis of colorectal adenocarcinoma to prostate and urinary bladder which was proven histopathologically. Despite the fact that this condition is uncommon in clinical practice, the possibility of metastatic involvement of prostate and urinary bladder should always be considered when the diagnosis of other primary carcinoma is made.
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