The CD4 helper T cells are the key players of cell mediated immunity. Upon antigenic interaction they show variable subsets depending upon specific cytokine profile. The development, maturation and differentiation of CD4 cells with other subtypes take place in well-coordinated manner. Such developmental processes influenced by thymic epithelial cells. Many scientists have suggested that the extirpation of salivary gland causes adverse effects on thymus gland, as this salivary gland considered as richest source of growth factors especially EGF(Epidermal Growth Factor). But the exact effect of this EGF on differentiation of thymocytes is unknown. So the present research work was carried out to find out the effect of salivary EGF on development, maturation and differentiation of CD4 cells. For this we have sialoadenectomised (removal of submandibular gland) and salivariadenectomised (removal of submandibular and sublingual glands) the 20 th day old male albino mice and maintained under normal conditions in the animal house along with control up to the age of ten weeks. There after the mice from both the groups were sacrificed; thymus was dissected out and subjected for immunofluorescence study of CD4 cell andCD4 cell count by flow cytometry. It has been observed that in sialoadenectomy and salivariadenectomy there was reduction in number of CD4 cells and degeneration of collagenous content of thymic epithelial network whiCh lead to disturbance in thymocytes maturation and selection, in particular CD4 helper T cells.

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Different honey samples i.e. Dabur, Himalaya, Patanjali and Madhuban pure organic honey; available in local market examined to study their antifungal property against different fungal strains including Candida albicans, Candida tropicalis and Rhizopus oryzae with Agar well diffusion method. In the present study all honey samples showed inhibitory activity against Candida albicans, In case of Candida tropicalis Dabur and Madhuban honey showed antifungal property. Surprisingly all honey sample failed to resists growth of Rhizopus oryzae. Such difference in inhibitory action may be due to variation in antimicrobial components, source of honey or processing and packaging of honey. In case of Rhizopus oryzae there may need of further dilution of honey samples. Most importantly all honey samples are best as natural remedy against Candida albicans associated infections or diseases.