In this study, Tongue fern (Pyrrosia lingua) plants that have been used traditionally as medicines. Their traditional medicinal uses, regions where indigenous people use the plants, parts of the plants used as medicines. This study was designed to assess the antioxidant and inhibition activities of extracts from P. lingua. In the P. lingua extracts was measured ethanol activity, 80.0% ethanol was high activity. The antioxidant activity was measured in 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-Azino-bis-(3ethylbenzothiazoline-6-sulfonic acid) (ABTS), assays. DPPH and ABTS radical in this experiment, solid and phenolic of extract were tested, but only an average concentration of 100 μg/mL was used. However, the phenolic extract is shown phenolic activity reached a peak. Also, phenolic extracts ware reached peak water and ethanol extracts. As a result, using the phenolic extracts did other antioxidant assays such as DPPH, ABTS, protection factor, and thiobarbituric acid reactive substances at 50-200 μg/mL concentrations. The activity of elastase and collagenase, inhibiting their activities may retard skin aging. α-Glucosidase and α-amylase, inhibitors need to be explored for the benefit of postprandial hyperglycemia in diabetic patients. Activities of tyrosinase, hyaluronidase and xanthine oxidase inhibitors of these enzymes are increasingly important ingredients in cosmetics and medications to protect the skin against hyperpigmentation and skin aging. Inhibition effects were investigated using the P. lingua extracts at 50-200 μg/mL concentrations. The expression levels of enzyme inhibitions activities were decrease in dependent-concentrations manner when P. lingua extracts were treated.

To test the potential use of the fern Pyrrosia lingua as an anti-inflammatory functional material, we examined the effects of P. lingua ethanol extract (PLE) on RAW 264.7 macrophages treated with the pro-inflammatory molecule lipopolysaccharide (LPS). Notably, up to 100 μg/mL PLE did not result in any discernable inhibition of cellular metabolic activity or cytotoxicity in the macrophages. However, supplementing LPS-treated RAW 264.7 macrophages with PLE significantly suppressed various pro-inflammatory responses in a dose-dependent manner, including i) phosphorylation of nuclear factor-kappa B (NF-κB) subunit p65; ii) accumulation of inducible nitric oxide synthase and cyclooxygenase-2; iii) expression of pro-inflammatory mediators, including prostaglandin E synthase 2 and nitrite; and iv) expression of pro-inflammatory biomarker genes, including interleukin 1 beta, interleukin 6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1. Taken together, our results indicate that PLE regulates NF-κB signaling and inhibits cytokine production. Therefore, the use of domestic biological resources like could be increased P. lingua as a novel functional material.

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