Identifying the components of Camellia oleifera honey and pollen and conducting corresponding toxicological tests are essential to revealing the mechanism of Camellia oleifera toxicity to honey bees. In this research, we investigated the saccharides and alkaloids in honey, nectar, and pollen from Camellia oleifera, which were compared with honey, nectar, and pollen from Brassica napus, a widely planted flowering plant. The result showed that melibiose, manninotriose, raffinose, stachyose, and lower amounts of santonin and caffeine were found in Camellia oleifera nectar, pollen, and honey but not in B. napus nectar, pollen, and honey. Toxicological experiments indicated that manninotriose, raffinose, and stachyose in Camellia oleifera honey are toxic to bees, while alkaloids in Camellia oleifera pollen are not toxic to honey bees. The toxicity mechanism of oligosaccharides revealed by temporal metabolic profiling is that oligosaccharides cannot be further digested by honey bees and thus get accumulated in honey bees, disturbing the synthesis and metabolism of trehalose, ultimately causing honey bee mortality.

Purpose To conduct a retrospective evaluation of a large clinical implementation of combined pulse oximeter (POX) and cardiac auscultation as a fast-screening device for congenital heart disease (CHD). Methods Every newborn in a large maternity healthcare center received auscultation and POX screening within 24 hours after delivery. When an abnormal heart murmur or SpO2 level was detected, an echocardiogram was ordered to confirm the diagnosis of CHD. Results From January 1, 2018 to December 31, 2019, there were 44,147 livebirths at the studied hospital where 498 suspected CHD were identified: 27 newborns by POX screening and 471 by cardiac auscultation. The diagnosis was further confirmed in 458 neonates through echocardiogram. This result put forth an overall diagnosis rate of 92.0%. Cardiac auscultation detected the majority of CHD cases 438 (95.6%) while POX only screened 20 (4.4%) cases. Interestingly, no CHD case was detected by both auscultation examination and POX screening. Auscultation detected most of the common types of CHD, but POX excelled in identifying rare and critical cases. POX screening alone had a very low accuracy of 74.07% in positive predict value (PPV). On the other hand, auscultation functioned well in terms of PPV and negative predict value (NPV) (92.99 and 99.95%, respectively), but the addition of POX improved the overall screening performance resulting in 100% NPV. We also validate the finding with the data 6 months after the study period. Conclusion Our study demonstrated that addition of pulse oximetry to routine cardiac auscultation could be used as an accurate and feasible screening for early screening of CHD in newborns in large-scale clinical practice.

Purpose To investigate the feasibility and reliability of pulse oximeter (POX) as a fast-screening device for congenital heart disease (CHD) through comparing performances of cardiac auscultation and POX in a large retrospective single center study. Methods Every newborn received auscultation and POX tests separately. When an abnormal murmur or SpO2 level is detected, an echocardiogram will be ordered to confirm the diagnosis of CHD in the newborn. Results From January 1, 2018 to December 31, 2019, there were 44,147 livebirths at the studied hospital where 498 newborns were diagnosed with CHD: 27 newborns by POX screening and 471 by cardiac auscultation. The diagnosis was further confirmed in 458 neonates through echocardiogram. This result puts forth an overall diagnosis rate of 91.97%. Cardiac auscultation detected the majority of CHD cases 438 (95.6%) while POX only screened 20 (4.4%) cases. Interestingly, no CHD case was detected by both auscultation examination and POX screening. Auscultation detected most of the common types of CHD, but POX excelled in identifying rare and critical cases. POX screening alone had a very low accuracy of 3.41% in positive predict value (PPV). On the other hand, auscultation can function well in terms of PPV and negative predict value (NPV) (92.99% and 99.95%, respectively), but the addition of POX could improve the overall screening performance and reach NPV to 100%. We also validate the finding with the data six months after the study period. Conclusion Auscultation screening operates well in detecting common CHDs whereas POX screening excels in distinguishing rare, yet critical, cases of CHDs. Therefore, an ideal CHD screening plan should combine cardiac auscultation and POX screening.