Candida albicans is a yeast normally found in the human microbiota, but it is considered opportunistic, causing a pathology called candidiasis. The essential oil of oregano (Origanum vulgare) has thymol and carvacrol in its composition, compounds with proven antimicrobial and antifungal action. Thus, the present study evaluated the Minimum Inhibitory Concentration (MIC) of essential oil of oregano on the strain ATCC 90028 of Candida albicans through macro and microdilution techniques, where it showed antifungal activity in vitro. Fungal suspensions were treated with different concentrations of essential oil (100, 50, 25, 12.5, 6.25, 3.125 and 1.56 uL/mL) where it was possible to identify the MIC's of 6.250 uL/mL and 12.5 uL /mL in the macrodilution and microdilution techniques, respectively. Thus, it is concluded that the essential oil of Origanum vulgare has a promising antifungal action, especially on Candida albicans, and, therefore, potential therapeutic action.